Capoluongo E, Moretto D, Giglio A, Belardi M, Prignano G, Crescimbeni E, Cordiali-Fei P, Maini A, DI Carlo A, Mercantini R, Giannetti A, Ameglio F
Laboratory of Microbiology, *Laboratory of Clinical Pathology, †Center for Sexually Transmitted Diseases, ‡Scientific Director of the Istituto S. Gallicano, Roma, Italy and Clinica Dermatologica of the University of Modena, Modena, Italy.
J Med Microbiol. 2000 Nov;49(11):985-991. doi: 10.1099/0022-1317-49-11-985.
Opportunist infections involving Candida albicans often develop in HIV-positive patients and oral lesions tend to become more frequent as the disease progresses. Previous studies have shown contrasting results concerning the variability of the pulsed-field gel electrophoresis (PFGE) subtypes of C. albicans observed in HIV-positive patients. Carriage of C. albicans was determined by an oral rinse technique; 41 strains of C. albicans (78% serotype A and 22% serotype B) were isolated. There was a direct correlation between candidal load (cfu/ml) and the blood HIV load, whereas there was an inverse correlation with the stage of disease and the CD4 cell counts. The PFGE patterns of isolates were variable with regard to the number and positions of bands. The variability of the band sizes in some run positions showed a Gaussian distribution. Generally, the most frequent size variants were associated with the strains with the highest cfu/ml and lowest CD4 counts (< or =200 cells/microl). These findings suggest a possible strain selection over time during disease progression, especially in HIV-positive subjects with low CD4 counts.
涉及白色念珠菌的机会性感染常发生于HIV阳性患者，且随着疾病进展，口腔病变往往更为频繁。先前的研究对于在HIV阳性患者中观察到的白色念珠菌脉冲场凝胶电泳（PFGE）亚型的变异性给出了相互矛盾的结果。白色念珠菌的携带情况通过口腔冲洗技术确定；分离出41株白色念珠菌（78%为A型血清型，22%为B型血清型）。念珠菌载量（cfu/ml）与血液中的HIV载量呈直接相关，而与疾病阶段和CD4细胞计数呈负相关。分离株的PFGE图谱在条带数量和位置方面存在差异。在某些电泳位置，条带大小的变异性呈现高斯分布。一般来说，最常见的大小变异与cfu/ml最高且CD4计数最低（≤200个细胞/微升）的菌株相关。这些发现表明在疾病进展过程中可能会随时间发生菌株选择，尤其是在CD4计数低的HIV阳性受试者中。
