Imbert-Bernard C, Valentin A, Reynes J, Mallié M, Bastide J M
Laboratoire de Parasitologie-Immunologie, Faculté de Pharmacie, Montpellier, France.
Eur J Clin Microbiol Infect Dis. 1994 Sep;13(9):711-6. doi: 10.1007/BF02276053.
The sensitivity of fluconazole, serotype and adherence to buccal epithelial cells of 46 Candida albicans isolates was studied in vitro. Strains were isolated from 18 HIV-positive patients with variable CD4+ lymphocyte counts who were receiving long-term fluconazole therapy. Results showed that sensitivity to fluconazole correlated with serotype. Of the sensitive isolates 81.5% were serotype A and 18.5% serotype B, whereas of the resistant isolates 37.5% were serotype A and 62.5% serotype B. Sensitivity to fluconazole also correlated with adherence capacity, resistant isolates showing slightly less adherence to buccal epithelial cells than sensitive isolates. Weakly adherent isolates represented 87% of resistant isolates and only 63% of sensitive isolates. Resistant strains were isolated in highly immunocompromised patients. All strains isolated in patients with CD4+ lymphocyte counts above 200 lymphocytes/microliters were sensitive, whereas intermediate and resistant strains were isolated in patients with counts below 200 lymphocytes/microliters. Statistical analyses showed no correlation between adherence of isolates to buccal epithelial cells and CD4+ lymphocyte counts.
体外研究了46株白色念珠菌分离株对氟康唑的敏感性、血清型以及对颊黏膜上皮细胞的黏附性。菌株从18例接受长期氟康唑治疗、CD4 +淋巴细胞计数各异的HIV阳性患者中分离得到。结果显示，对氟康唑的敏感性与血清型相关。敏感分离株中81.5%为A血清型，18.5%为B血清型；而耐药分离株中37.5%为A血清型，62.5%为B血清型。对氟康唑的敏感性还与黏附能力相关，耐药分离株对颊黏膜上皮细胞的黏附性略低于敏感分离株。弱黏附性分离株在耐药分离株中占87%，在敏感分离株中仅占63%。耐药菌株在免疫功能极度低下的患者中分离得到。CD4 +淋巴细胞计数高于200个/微升的患者所分离出的所有菌株均敏感，而计数低于200个/微升的患者中分离出了中度和耐药菌株。统计分析表明，分离株对颊黏膜上皮细胞的黏附性与CD4 +淋巴细胞计数之间无相关性。
