Brown Nat F, Beacham Ifor R
School of Health Science, Griffith University, Gold Coast Campus, Southport, Queensland 4217, Australia.
J Med Microbiol. 2000 Nov;49(11):993-1001. doi: 10.1099/0022-1317-49-11-993.
Melioidosis is an infectious disease caused by Burkholderia pseudomallei. Genomic subtractive hybridisation was performed with the closely related avirulent species B. thailandensis to identify virulence genes of B. pseudomallei. The subtractive hybridisation products were highly specific for B. pseudomallei. Sequence analysis revealed a number of putative virulence factors, as well as apparently novel sequences of unknown function. The subtracted library contained DNA regions of relatively low G + C mol% content, which were distributed throughout the B. pseudomallei genome. The distribution of subtracted sequences amongst a collection of 22 B. pseudomallei isolates was found to be variable, with the exception of three strains which almost universally lacked the subtracted sequences. These three strains also differed in that they were highly haemolytic, indicating a possible separate virotype.
类鼻疽是由类鼻疽伯克霍尔德菌引起的一种传染病。利用与之密切相关的无毒菌株泰国伯克霍尔德菌进行基因组消减杂交，以鉴定类鼻疽伯克霍尔德菌的毒力基因。消减杂交产物对类鼻疽伯克霍尔德菌具有高度特异性。序列分析揭示了一些假定的毒力因子以及功能未知的明显新序列。消减文库包含G + C摩尔百分比含量相对较低的DNA区域，这些区域分布在类鼻疽伯克霍尔德菌基因组中。发现在22株类鼻疽伯克霍尔德菌分离株中，消减序列的分布是可变的，但有三株菌株几乎普遍缺乏消减序列除外。这三株菌株的不同之处还在于它们具有高度溶血活性，表明可能是一种单独的病毒型。
