酿酒酵母中含聚腺苷酸核糖核酸的周转
Turnover of polyadenylate-containing ribonucleic acid in Saccharomyces cerevisiae.
作者信息
Hynes N E, Phillips S L
出版信息
J Bacteriol. 1976 Feb;125(2):595-600. doi: 10.1128/jb.125.2.595-600.1976.
Abstract
We examined the kinetics of incorporation of [3H]adenine into polyadenylate-containing ribonucleic acid [poly(A)-containing RNA] in yeast. The total poly(A)-containing RNA from spheroplasts and intact cells and the polysomal poly(A)-containing RNA exhibited similar incorporation kinetics. At 30 C half-saturation of the pool of poly(A)-containing RNA with label occurred in approximately 22 min. Since precursor pools appeared to require 5 min to saturate with label, we conclude that at 30 C messenger RNA molecules in yeast decay with an average half-life of 17 min.
摘要
我们研究了[3H]腺嘌呤掺入酵母中含聚腺苷酸的核糖核酸（含poly(A)的RNA）的动力学。来自原生质球和完整细胞的总含poly(A)的RNA以及多核糖体含poly(A)的RNA表现出相似的掺入动力学。在30℃时，含poly(A)的RNA池被标记半饱和大约发生在22分钟。由于前体池似乎需要5分钟才能被标记饱和，我们得出结论，在30℃时，酵母中的信使RNA分子平均半衰期为17分钟。
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本文引用的文献
1
Macromolecule synthesis in yeast spheroplasts.酵母原生质体中的大分子合成。
J Bacteriol. 1967 Nov;94(5):1697-705. doi: 10.1128/jb.94.5.1697-1705.1967.
2
Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes.未成熟鸭红细胞中的巨大快速标记核糖核酸和细胞质信使核糖核酸
J Mol Biol. 1966 Sep;20(1):145-82. doi: 10.1016/0022-2836(66)90123-9.
3
Erythroid cell-development in fetal mice: synthetic capacity for different proteins.胎鼠红细胞的发育：不同蛋白质的合成能力
J Mol Biol. 1968 Apr 14;33(1):79-91. doi: 10.1016/0022-2836(68)90282-9.
4
The turnover of nuclear DNA-like RNA in HeLa cells.海拉细胞中类核DNA的核糖核酸周转
J Cell Biol. 1968 Oct;39(1):112-8. doi: 10.1083/jcb.39.1.112.
5
The effect of cycloheximide upon polyribosome stability in two yeast mutants defective respectively in the initiation of polypeptide chains and in messenger RNA synthesis.放线菌酮对两个分别在多肽链起始和信使核糖核酸合成方面存在缺陷的酵母突变体中多核糖体稳定性的影响。
Mol Gen Genet. 1970;106(4):347-61. doi: 10.1007/BF00324052.
6
Selective measurement of the synthesis and metabolic stability of messenger RNA in 3T3 mouse cells.3T3小鼠细胞中信使核糖核酸合成与代谢稳定性的选择性测量。
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7
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9
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