酿酒酵母S-腺苷甲硫氨酸池的标记动力学
Kinetics of labeling of the S-adenosylmethionine pool of Saccharomyces cerevisiae.
作者信息
Warner J R, Morgan S A, Shulman R W
出版信息
J Bacteriol. 1976 Mar;125(3):887-91. doi: 10.1128/jb.125.3.887-891.1976.
Abstract
A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine is reduced by half within 20 s. During that period there is a substantial expansion of the pool. A logarithmically growing cell in synthetic medium contains about 2 X 10(6) molecules of S-adenosylmethionine, of which only 10% is used for the methylation of ribonucleic acid molecules.
摘要
已开发出一种用于研究酵母中S-腺苷甲硫氨酸池比活性的方法。加入[甲基-³H]甲硫氨酸后30秒内，该池达到最大比活性的一半。加入过量非放射性甲硫氨酸后，S-腺苷甲硫氨酸的比活性在20秒内降低一半。在此期间，该池有显著扩张。在合成培养基中对数生长的细胞含有约2×10⁶个S-腺苷甲硫氨酸分子，其中仅10%用于核糖核酸分子的甲基化。
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