一种用于检测豚鼠促有丝分裂因子的微孔板培养方法。
A microplate culture method for assay of guinea pig mitogenic factor.
作者信息
Ashworth L A, Ford W H
出版信息
J Immunol Methods. 1975 Nov;9(1):47-57. doi: 10.1016/0022-1759(75)90034-4.
PMID:1107428
Abstract
An improved method for assay of guinea pig mitogenic factor (MF) is described. The technique is based on the use of thymocytes in microplate cultures and yields significant savings in time, labour and materials. The quantitative potential of the assay is examined using antigen- and concanavalin A-indiced MF, and its application to assessing in vitro immune responsiveness of individual guinea pigs is explored.
摘要
本文描述了一种改进的豚鼠促有丝分裂因子（MF）检测方法。该技术基于在微孔板培养中使用胸腺细胞，可显著节省时间、人力和材料。使用抗原和刀豆蛋白A诱导的MF检测该方法的定量潜力，并探索其在评估个体豚鼠体外免疫反应性中的应用。
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