Goldschmidt M C, DuPont H L
J Infect Dis. 1976 Feb;133(2):153-6. doi: 10.1093/infdis/133.2.153.
Forty-eight strains of Escherichia coli isolated from children with diarrhea were classified according to nine enteropathogenic serotypes. The strains were examined for production of enterotoxin and for invasiveness by study of bacteria or bacteria-free filtrates in conventional animal and tissue culture models. Filtrates of only three strains (6%) consistently dilated rabbit ileal loops, while all 48 strains yielded negative results in suckling mice, adrenal cells, and guinea pig eyes. When filtrates of the three strains that dilated the rabbit ileum were heated at 60 C for 30 min, the reaction in rabbit ileal loops was negative; this finding indicated the production of a heat-labile enterotoxin. This study shows the lack of correlation between classical enteropathogenic serotypes of E. coli and presently known virulence properties in animal models. The results raise doubts about the value of serotyping E. coli isolates from sporadic cases of diarrhea. When it is suspected that an E. coli isolate is enteropathogenic, it may be important to perform more than one laboratory assay.
从腹泻患儿中分离出的48株大肠杆菌，根据9种肠道致病血清型进行了分类。通过在传统动物和组织培养模型中研究细菌或无细菌滤液，检测这些菌株产生肠毒素的情况以及侵袭性。只有3株菌株（6%）的滤液能持续使兔回肠袢扩张，而所有48株菌株在乳鼠、肾上腺细胞和豚鼠眼中均产生阴性结果。当使兔回肠扩张的3株菌株的滤液在60℃加热30分钟后，兔回肠袢中的反应变为阴性；这一发现表明产生了一种不耐热肠毒素。本研究表明，大肠杆菌的经典肠道致病血清型与动物模型中目前已知的毒力特性之间缺乏相关性。这些结果对散发性腹泻病例中大肠杆菌分离株血清分型的价值提出了质疑。当怀疑大肠杆菌分离株具有肠道致病性时，进行不止一种实验室检测可能很重要。
