Duda G N, Haisch A, Endres M, Gebert C, Schroeder D, Hoffmann J E, Sittinger M
Charité, Campus Virchow-Klinikum, Humboldt University of Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany.
J Biomed Mater Res. 2000;53(6):673-7. doi: 10.1002/1097-4636(2000)53:6<673::aid-jbm9>3.0.co;2-v.
Traumatic events are a primary cause for local lesions of articular cartilage. If treated early, restoration of the initial joint geometry and integrity may be achieved. In large defects, sufficient material is not available to bridge the affected area. Heterologeous transplantation is not well accepted due to the risk of infection and immune response. Alternatives are cartilage-like structures, which may be cultured in vitro and transplanted into the defect site. Critical to the success of these new tissues are their mechanical properties. Goals of this study were to generate a hyaline-like cartilage structure, to evaluate its performance in vivo and to verify that its cellular and material properties meet those of native cartilage. Hyaline-like cartilage specimens were generated in vitro and implanted in the backs of nude mice. Specimens were explanted after 6 and 12 weeks, mechanically tested using an indentation test and histologically examined. In mechanical testing, stiffness and failure load significantly increased between weeks 6 and 12. At 12 weeks, mechanical properties of the hyaline-like cartilage were comparable to those of native nasal septal cartilage. Compared to native articular cartilage, the engineered tissue achieved up to 30-50% in strength and mechanical stiffness. In histological examination, specimens showed neocartilage formation. The mechanical testing procedure proved to be sufficiently sensitive to identify differences in properties between cartilage specimens of different origin and at different stages of healing. As an adjunct to histological analysis, mechanical testing may be a valuable tool for judging the utility of engineered cartilage prior to a broad clinical usage.
创伤性事件是关节软骨局部损伤的主要原因。如果早期治疗，可实现初始关节几何形状和完整性的恢复。在大面积缺损中，没有足够的材料来桥接受影响区域。由于感染风险和免疫反应，异种移植未被广泛接受。替代方法是培养可在体外培养并移植到缺损部位的软骨样结构。这些新组织成功的关键在于其力学性能。本研究的目的是生成类似透明软骨的结构，评估其在体内的性能，并验证其细胞和材料特性是否符合天然软骨的特性。体外生成类似透明软骨的标本并植入裸鼠背部。6周和12周后取出标本，使用压痕试验进行力学测试并进行组织学检查。在力学测试中，6周和12周之间刚度和破坏载荷显著增加。在12周时，类似透明软骨的力学性能与天然鼻中隔软骨相当。与天然关节软骨相比，工程组织的强度和力学刚度提高了30%-50%。在组织学检查中，标本显示有新软骨形成。力学测试程序被证明对识别不同来源和不同愈合阶段软骨标本的性能差异足够敏感。作为组织学分析的辅助手段，力学测试可能是在广泛临床应用之前判断工程软骨效用的有价值工具。
