Pérez S E, Yáñez J, Marín O, Anadón R, González A, Rodríguez-Moldes I
Department of Cell and Molecular Biology, Faculty of Sciences, University of A Coruña, 15071-A Coruña, Spain.
J Comp Neurol. 2000 Dec 18;428(3):450-74. doi: 10.1002/1096-9861(20001218)428:3<450::aid-cne5>3.0.co;2-t.
The distribution of cholinergic neurons and fibers was studied in the brain and rostral spinal cord of the brown trout and rainbow trout by using an antiserum against the enzyme choline acetyltransferase (ChAT). Cholinergic neurons were observed in the ventral telencephalon, preoptic region, habenula, thalamus, hypothalamus, magnocellular superficial pretectal nucleus, optic tectum, isthmus, cranial nerve motor nuclei, and spinal cord. In addition, new cholinergic groups were detected in the vascular organ of the lamina terminalis, the parvocellular and magnocellular parts of the preoptic nucleus, the anterior tuberal nucleus, and a mesencephalic tegmental nucleus. The presence of ChAT in the magnocellular neurosecretory system of trout suggests that acetylcholine is involved in control of hormone release by neurosecretory terminals. In order to characterize the several cholinergic nuclei observed in the isthmus of trout, their projections were studied by application of 1,1;-dioctadecyl-3,3,3;, 3;-tetramethylindocarbocyanine perchlorate (DiI) to selected structures of the brain. The secondary gustatory nucleus projected mainly to the lateral hypothalamic lobes, whereas the nucleus isthmi projected to the optic tectum and parvocellular superficial pretectal nucleus, as previously described in other teleost groups. In addition, other isthmic cholinergic nuclei of trout may be homologs of the mesopontine system of mammals. We conclude that the cholinergic systems of teleosts show many primitive features that have been preserved during evolution, together with characteristics exclusive to the group.
利用抗胆碱乙酰转移酶（ChAT）的抗血清，研究了褐鳟和虹鳟脑及吻侧脊髓中胆碱能神经元和纤维的分布。在腹侧端脑、视前区、缰核、丘脑、下丘脑、大细胞浅层顶盖前核、视顶盖、峡部、脑神经运动核和脊髓中观察到胆碱能神经元。此外，在终板血管器、视前核的小细胞和大细胞部分、前结节核以及中脑被盖核中检测到新的胆碱能组。鳟鱼大细胞神经分泌系统中ChAT的存在表明乙酰胆碱参与神经分泌终末对激素释放的控制。为了表征在鳟鱼峡部观察到的几个胆碱能核，通过将1,1'-二辛基-3,3,3',3'-四甲基吲哚羰花青高氯酸盐（DiI）应用于脑的选定结构来研究它们的投射。次级味觉核主要投射到下丘脑外侧叶，而峡核投射到视顶盖和小细胞浅层顶盖前核，如先前在其他硬骨鱼群中所描述的。此外，鳟鱼峡部的其他胆碱能核可能是哺乳动物脑桥中脑系统的同源物。我们得出结论，硬骨鱼的胆碱能系统显示出许多在进化过程中保留下来的原始特征，以及该类群特有的特征。
