Ayana A, Bekele E, Bryngelsson T
Addis Ababa University, Faculty of Science, Department of Biology, Ethiopia.
Hereditas. 2000;132(3):249-54. doi: 10.1111/j.1601-5223.2000.t01-1-00249.x.
The extent and distribution of genetic variation in wild sorghum (Sorghum bicolor ssp. verticilliflorum (L.) Moench) collected from five different geographical regions in Ethiopia were analyzed using random amplified polymorphic DNA (RAPD) markers for 93 individuals representing 11 populations. Nine decamer primers generated a total of 83 polymorphic bands with 8-12 bands per primer and a mean of 9 bands across the 93 individuals. The amount of genetic variation among the populations (H = 0.37) and among the geographical region (H = 0.44) was low to moderate, despite the high degree of polymorphic bands per primer. Similarly, the mean genetic distance (0.08) among populations as well as among regions of origin (0.04) of the population was found to be low. The low genetic variation may be due to the reduced population size of the wild sorghum in Ethiopia because of habitat change. Partitioning of the genetic variation into between and within the population as well as between and within the regions of origin revealed that 75% and 88% of the variation was found within the populations and within the regions, respectively. Cluster analysis of genetic distance estimates further confirmed low level of differentiation of wild sorghum populations both on population and regional bases. The implications of the results for genetic conservation purposes are discussed.
利用随机扩增多态性DNA（RAPD）标记，对来自埃塞俄比亚五个不同地理区域的野生高粱（双色高粱轮生亚种（L.）Moench）进行遗传变异程度和分布分析，研究对象为代表11个种群的93个个体。九条十聚体引物共产生了83条多态性条带，每条引物产生8 - 12条带，93个个体平均每条引物产生9条带。尽管每条引物的多态性条带程度较高，但种群间（H = 0.37）和地理区域间（H = 0.44）的遗传变异量较低至中等。同样，种群间以及种群起源区域间的平均遗传距离（0.08和0.04）也较低。遗传变异低可能是由于埃塞俄比亚野生高粱因栖息地变化导致种群规模减小。将遗传变异划分为种群间和种群内以及起源区域间和区域内，结果显示分别有75%和88%的变异存在于种群内和区域内。基于遗传距离估计的聚类分析进一步证实，野生高粱种群在种群和区域层面的分化程度较低。本文还讨论了这些结果对遗传保护目的的意义。
