Longman R J, Douthwaite J, Sylvester P A, Poulsom R, Corfield A P, Thomas M G, Wright N A
University Department of Surgery, Bristol Royal Infirmary, Bristol BS2 8HW, UK.
Gut. 2000 Dec;47(6):792-800. doi: 10.1136/gut.47.6.792.
Trefoil factor family (TFF) peptides and the chromosome 11p15.5 mucin glycoproteins are expressed and secreted in a site specific fashion along the length of the gastrointestinal tract. Evidence for coexpression of mucins and trefoil peptides has been suggested in numerous gastrointestinal mucosal pathologies. The ulcer associated cell lineage (UACL) occurs at sites of chronic ulceration in Crohn's disease, expresses all three trefoil peptides, and is implicated in mucosal restitution. We tested the hypothesis that individual trefoil peptides are uniquely localised with specific mucins in the UACL and normal gastrointestinal epithelia.
Expression of mucin genes in the UACL from small bowel tissue of patients with Crohn's disease was detected by in situ hybridisation, and localisation of the products by immunohistochemistry. Colocalisation of mucins and trefoil peptides was demonstrated by immunofluorescent colabelling in UACL and normal gastrointestinal epithelia.
MUC5AC and TFF1 were colocalised in distal ductular and surface elements of the UACL and in foveolar cells of the stomach, whereas MUC6 and TFF2 were colocalised to acinar and proximal ductular structures in the UACL, in the fundus and deep antral glands of the stomach, and in Brunner's glands of the duodenum. MUC5B was found sporadically throughout the UACL and gastric body. MUC2 was absent from the UACL, Brunner's glands, and stomach. MUC2 and TFF3 were colocalised throughout the large and small bowel mucosa.
The UACL has a unique profile of mucin gene expression. Coordinated localisation of trefoil peptides and mucins in UACL and normal gastrointestinal epithelia suggests they may assist each others' functions in protection and repair of gastrointestinal mucosa.
三叶因子家族（TFF）肽和11号染色体p15.5黏蛋白糖蛋白沿胃肠道全长以位点特异性方式表达和分泌。在多种胃肠道黏膜病变中已提示存在黏蛋白和三叶肽共表达的证据。溃疡相关细胞谱系（UACL）出现在克罗恩病的慢性溃疡部位，表达所有三种三叶肽，并与黏膜修复有关。我们检验了以下假设：在UACL和正常胃肠道上皮中，单个三叶肽与特定黏蛋白独特地定位在一起。
通过原位杂交检测克罗恩病患者小肠组织UACL中黏蛋白基因的表达，并通过免疫组织化学对产物进行定位。通过UACL和正常胃肠道上皮中的免疫荧光共标记证明黏蛋白和三叶肽的共定位。
MUC5AC和TFF1在UACL的远端导管和表面成分以及胃小凹细胞中共定位，而MUC6和TFF2在UACL的腺泡和近端导管结构、胃底和胃窦深部腺体以及十二指肠的布伦纳腺中共定位。MUC5B在整个UACL和胃体中散在发现。UACL、布伦纳腺和胃中不存在MUC2。MUC2和TFF3在整个大肠和小肠黏膜中共定位。
UACL具有独特的黏蛋白基因表达谱。UACL和正常胃肠道上皮中三叶肽与黏蛋白的协同定位表明，它们可能在胃肠道黏膜的保护和修复中相互协助发挥功能。
