Hanby A M, Pera M, Filipe I, Duranceau A, Wright N A, Pera M, Grande L, Poulsom R
Clinical Oncology Unit, Guys Hospital, London, United Kingdom.
Am J Pathol. 1997 Dec;151(6):1819-24.
We have studied the histological changes observed in the mucosa of 10 rats in the region of a esophagojejunostomy to evaluate it as a model for the ulcer-associated cell lineage (UACL). In man, the UACL has a distinctive morphology, proliferative organization, and pattern of trefoil peptide localization. We have therefore examined these aspects aided by immunohistochemistry and in situ hybridization to the trefoil peptides TFF1, TFF2, and TFF3. Only TFF2 was studied by immunohistochemistry, whereas the mRNAs for all three peptides were examined by in situ hybridization using 35S-labeled riboprobes. The marker MIB-1 to the Ki67 proliferation-related antigen was used to examine the proliferative organization of UACL-like changes. In all cases, columnar epithelialization of the distal esophagus was seen, and in all, glands with morphological and gene expression attributes of the UACL were identified. TFF3 mRNA localized patchily throughout the UACL, whereas TFF1 mRNA was found in the upper portions of the lineage and TFF2 mRNA and its product in the acini. These lineages showed virtually no intrinsic proliferative activity. These appearances are similar to those seen in early human UACL, and we therefore propose this that this represents the first published animal model of this lineage.
我们研究了10只大鼠食管空肠吻合术区域黏膜的组织学变化，以评估其作为溃疡相关细胞谱系（UACL）模型的可行性。在人类中，UACL具有独特的形态、增殖组织和三叶肽定位模式。因此，我们借助免疫组织化学和针对三叶肽TFF1、TFF2和TFF3的原位杂交技术对这些方面进行了研究。免疫组织化学仅研究了TFF2，而使用35S标记的核糖探针通过原位杂交检测了所有三种肽的mRNA。使用针对Ki67增殖相关抗原的标记物MIB-1来检查UACL样变化的增殖组织。在所有病例中，均可见远端食管柱状上皮化生，并且均鉴定出具有UACL形态和基因表达特征的腺体。TFF3 mRNA在整个UACL中呈散在分布，而TFF1 mRNA见于该谱系的上部，TFF2 mRNA及其产物见于腺泡。这些谱系几乎没有内在增殖活性。这些表现与早期人类UACL所见相似，因此我们认为这是该谱系首个发表的动物模型。
