Ellies M, Laskawi R, Tormählen G, Götz W
Department of Otorhinolaryngology, University of Göttingen, Germany.
J Oral Maxillofac Surg. 2000 Nov;58(11):1251-6. doi: 10.1053/joms.2000.16625.
In this investigation, the effect of a local injection of botulinum toxin A on the concentration of acetylcholinesterase in the parotid gland of the rat was examined.
After local injection into the parotid glands of female Wistar rats, the treated glands were excised, and immunohistochemical staining for acetylcholinesterase was performed. To discover possible changes in cell morphology after local application of botulinum toxin A, morphometric measurements also were performed on the excised parotid glands.
In contrast to the untreated, physiologic saline-injected, glands, there was a decrease in the concentration of acetylcholinesterase in the glands treated with botulinum toxin. No persistent changes in the number of acinar cells could be observed.
Because the cholinergic pathway of the autonomic nervous system has great importance in the secretion of fluid from the salivary glands, blocking this pathway and local application of botulinum toxin offers a possible therapeutic option for the treatment of hypersalivation in various otolaryngologic and neurologic diseases.
在本研究中，检测了局部注射A型肉毒杆菌毒素对大鼠腮腺中乙酰胆碱酯酶浓度的影响。
对雌性Wistar大鼠的腮腺进行局部注射后，切除处理过的腺体，并进行乙酰胆碱酯酶的免疫组织化学染色。为了发现局部应用A型肉毒杆菌毒素后细胞形态可能发生的变化，还对切除的腮腺进行了形态计量学测量。
与未处理的、注射生理盐水的腺体相比，用肉毒杆菌毒素处理的腺体中乙酰胆碱酯酶浓度降低。未观察到腺泡细胞数量的持续变化。
由于自主神经系统的胆碱能途径在唾液腺分泌液体中具有重要作用，阻断该途径并局部应用肉毒杆菌毒素为治疗各种耳鼻喉科和神经科疾病中的流涎过多提供了一种可能的治疗选择。
