Jung E, Hoogland C, Chiappe D, Sanchez J C, Hochstrasser D F
Central Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland.
Electrophoresis. 2000 Oct;21(16):3483-7. doi: 10.1002/1522-2683(20001001)21:16<3483::AID-ELPS3483>3.0.CO;2-X.
This short communication describes the establishment of a two-dimensional electrophoresis (2-DE) reference map of nuclear proteins isolated from human liver. The human liver nuclei 2-DE reference map contains 1497 spots. In an initial identification study using peptide mass fingerprinting as a means of protein identification we were able to identify 26 spots corresponding to 15 different proteins. The human liver nuclei 2-DE reference map is now included in the SWISS-2DPAGE database, which can be accessed through the ExPASy server (http://www.expasy.ch/ch2d/).
本简短通讯描述了从人肝脏中分离得到的核蛋白二维电泳（2-DE）参考图谱的建立。人肝脏细胞核2-DE参考图谱包含1497个斑点。在一项使用肽质量指纹图谱作为蛋白质鉴定手段的初步鉴定研究中，我们能够鉴定出与15种不同蛋白质相对应的26个斑点。人肝脏细胞核2-DE参考图谱现已收录于SWISS-2DPAGE数据库，可通过ExPASy服务器（http://www.expasy.ch/ch2d/）访问。
