Sanchez J C, Golaz O, Frutiger S, Schaller D, Appel R D, Bairoch A, Hughes G J, Hochstrasser D F
Clinical Chemistry Laboratory, Geneva Hospital, Switzerland. sanchez/dim.hcuge.CH
Electrophoresis. 1996 Mar;17(3):556-65. doi: 10.1002/elps.1150170326.
The systematic sequencing of the yeast genome will soon be completed. A new challenge has been launched by the EUROFAN (European Functional Analysis) project whose goal is to elucidate the physiological and biochemical function of newly discovered open reading frames (ORF) from yeast. One of the approaches is to use protein-based technologies such as two-dimensional gel electrophoresis and protein identification in order to establish a yeast reference map. Modified protein patterns can be compared to the reference map which hopefully will help identify changes related, for example, to growth processes or developmental events. This paper describes the yeast SWISS-2DPAGE database in which charge separation was obtained using immobilized pH gradient (IPG). Proteins identified by gel comparison, amino acid composition analysis and/or microsequencing are recorded and described in an accessible uniform format. We have identified more than one hundred polypeptides, several of which were newly mapped. In addition, the yeast SWISS-2DPAGE database can be freely accessed through the World Wide Web (WWW) network on the ExPASy molecular biology server.
酵母基因组的系统测序即将完成。欧洲功能分析（EUROFAN）项目发起了一项新挑战，其目标是阐明酵母中新发现的开放阅读框（ORF）的生理和生化功能。其中一种方法是使用基于蛋白质的技术，如二维凝胶电泳和蛋白质鉴定，以建立酵母参考图谱。可以将修饰后的蛋白质图谱与参考图谱进行比较，这有望有助于识别例如与生长过程或发育事件相关的变化。本文描述了酵母SWISS-2DPAGE数据库，其中使用固定化pH梯度（IPG）进行电荷分离。通过凝胶比较、氨基酸组成分析和/或微量测序鉴定的蛋白质以可访问的统一格式进行记录和描述。我们已经鉴定出一百多种多肽，其中几种是新定位的。此外，酵母SWISS-2DPAGE数据库可以通过ExPASy分子生物学服务器上的万维网（WWW）网络免费访问。
