Floccari M E, Carranza M M, Parada J L
Departamento de Química Biológica, Facultad de Ciencas Exactas y Naturales, Universidad de Buenos Aires, Argentina.
J Food Prot. 2000 Nov;63(11):1591-3. doi: 10.4315/0362-028x-63.11.1591.
To evaluate the presence of Yersinia enterocolitica in raw chicken, 70 samples of chicken carcasses were obtained from a Buenos Aires, Argentina, processing plant. The detection of this psychotropic microorganism was carried out using the whole carcass rinse method and enrichment in phosphate-buffered saline, 0.067 M, pH 7.6, at 4 degrees C, followed by alkaline treatment and isolation on cefsulodin-irgasan-novobiocin nutrient agar. Y. enterocolitica or related species were detected in 7 of 70 samples. From them, 4.3% were identified as Y. enterocolitica, 1.4% as Yersinia intermedia, and 4.3% as Yersinia frederiksenii. The serotype and phagotype of Y. intermedia isolates were O:5(2), 5(3), and Xz and those of Y. frederiksenii were O:4.16 and Xo. All Y. enterocolitica isolates belong to the biogroup 1A, serotype O:5, phagotype Xz, which presents uncertain pathogenic potential. These findings reinforce the worldwide concern on the microbiological quality of food products. Quality assurance programs on the whole poultry process are increasingly being adopted in Argentina.
为评估生鸡肉中是否存在小肠结肠炎耶尔森菌，从阿根廷布宜诺斯艾利斯的一家加工厂采集了70份鸡胴体样本。采用全胴体冲洗法并在4℃的0.067M、pH7.6的磷酸盐缓冲盐水中富集，随后进行碱性处理，并在头孢磺啶-伊曲康唑-新生霉素营养琼脂上进行分离，以此检测这种嗜冷微生物。在70份样本中的7份中检测到了小肠结肠炎耶尔森菌或相关菌种。其中，4.3%被鉴定为小肠结肠炎耶尔森菌，1.4%为中间型耶尔森菌，4.3%为费氏耶尔森菌。中间型耶尔森菌分离株的血清型和噬菌体型分别为O:5(2)、5(3)和Xz，费氏耶尔森菌的血清型和噬菌体型分别为O:4.16和Xo。所有小肠结肠炎耶尔森菌分离株均属于生物群1A，血清型为O:5，噬菌体型为Xz，其致病潜力尚不确定。这些发现强化了全球对食品微生物质量的关注。阿根廷越来越多地采用全禽加工过程中的质量保证计划。
