Eiss J
Scand J Infect Dis. 1975;7(4):249-51. doi: 10.3109/inf.1975.7.issue-4.05.
Stool specimens from patients with suspected Yersinia enterocolitica (Y. ent.) infection were cultured at +4 degrees C in a nutrient broth for 5 days and at the same time the same stool specimens were cultured by the routine method of the laboratory. The routine method gave 34 Y. ent. isolations. Selective culturing at +4 degrees C succeeded in isolation of the same 34 Y. ent. strains but also 15 further strains or a 44% increase in yield. Serial dilutions in broth of 34 strains of Y. ent., 7 strains of Y. pseudotuberculosis and 14 strains of other gram-negative enterobacteria were cultured at +4 degrees C. Colony counts were performed from each dilution after 2 and 5 days. Human pathogenic strains of Y. ent. (serotype 3, 8, 9) increased markedly at +4 degrees C in contrast to the other gram-negative enterobacteria.
对疑似小肠结肠炎耶尔森菌（Y. ent.）感染患者的粪便标本，在4℃下于营养肉汤中培养5天，同时用实验室常规方法对相同粪便标本进行培养。常规方法分离出34株Y. ent.。在4℃下进行选择性培养成功分离出相同的34株Y. ent.菌株，还另外分离出15株菌株，产量增加了44%。将34株Y. ent.、7株假结核耶尔森菌和14株其他革兰氏阴性肠道杆菌在肉汤中进行系列稀释后，于4℃下培养。在培养2天和5天后对每个稀释度进行菌落计数。与其他革兰氏阴性肠道杆菌相比，人致病性Y. ent.菌株（血清型3、8、9）在4℃下显著增加。
