培养的果蝇细胞中细胞质肌动蛋白的前体。
A precursor of cytoplasmic actin in cultured Drosophila cells.
作者信息
Berger E, Cox G
出版信息
J Cell Biol. 1979 Jun;81(3):680-3. doi: 10.1083/jcb.81.3.680.
Abstract
In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel electrophoresis, it has been found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated to be approximately 50 min.
摘要
在用[35S]甲硫氨酸进行25至120分钟的脉冲标记后，在连续培养的果蝇细胞系中检测到两种形式的细胞质肌动蛋白，分别命名为II型和III型。然而，这些肌动蛋白中只有II型在细胞中积累。通过使用脉冲追踪实验方案和二维凝胶电泳，发现肌动蛋白III型是更稳定的细胞质肌动蛋白II型的前体。肌动蛋白III型的半衰期估计约为50分钟。
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