Hamza-Chaffai A, Amiard J C, Pellerin J, Joux L, Berthet B
Université de Sfax, IPEIS, Sfax, Tunisia.
Comp Biochem Physiol C Toxicol Pharmacol. 2000 Sep;127(2):185-97. doi: 10.1016/s0742-8413(00)00147-x.
This work aimed to validate the relationship between metallothioneins (MTs) and metals (Cd, Cu and Zn) in field conditions. Specimens of the marine bivalve Ruditapes decussatus (Linné, 1758) from Gargour were transferred in two sites: Gargour and Sidi Mansour, both situated along the south-eastern coast of Tunisia. The bivalves were removed from pairs of cages at day 0 (date of transplantation), day 62 and day 132. Metals (Cd, Cu and Zn) and MTs were determined in the subcellular fractions of the digestive gland. In Gargour, metal and MT levels increased significantly after 62 days of transplantation. However, they showed modest and non-significant variations in Sidi Mansour. Zn was mainly associated with the insoluble fraction, whereas Cd and Cu percentages in the soluble and the insoluble fractions were equivalent. Simple correlation analysis showed a positive and significant relationship between MTs and each metal. If all metals were taken together, multiple correlations showed that MTs were significantly correlated with Cd and Zn, with an important coefficient for Cd, but no significant relationship was observed for Cu. Gel filtration chromatography showed that in the heat stable fraction, the only cytosolic SH rich compounds have an apparent low molecular mass (about 15 kDa), which could correspond to metallothioneins. In the digestive gland of R. decussatus MTs responded to moderate increases of metal contamination, without interference with other factors, and could be a promising biochemical indicator of metal exposure.
这项工作旨在验证野外条件下金属硫蛋白（MTs）与金属（镉、铜和锌）之间的关系。来自加尔古尔的海洋双壳贝类波纹巴非蛤（林奈，1758年）样本被转移到两个地点：加尔古尔和西迪·曼苏尔，这两个地点都位于突尼斯东南海岸。在第0天（移植日期）、第62天和第132天从成对的笼子中取出双壳贝类。测定消化腺亚细胞组分中的金属（镉、铜和锌）和金属硫蛋白。在加尔古尔，移植62天后金属和金属硫蛋白水平显著增加。然而，它们在西迪·曼苏尔显示出适度且不显著的变化。锌主要与不溶部分相关，而镉和铜在可溶和不溶部分中的百分比相当。简单相关分析表明金属硫蛋白与每种金属之间存在正相关且显著的关系。如果将所有金属一起考虑，多重相关分析表明金属硫蛋白与镉和锌显著相关，镉的系数较大，但未观察到与铜有显著关系。凝胶过滤色谱显示，在热稳定部分，唯一富含胞质巯基的化合物具有明显较低的分子量（约15 kDa），这可能对应于金属硫蛋白。在波纹巴非蛤的消化腺中，金属硫蛋白对金属污染的适度增加有反应，不受其他因素干扰，可能是一种有前景的金属暴露生化指标。
