Boyle S P, Dobson V L, Duthie S J, Hinselwood D C, Kyle J A, Collins A R
Rowett Research Institute, Bucksburn, Aberdeen, Scotland.
Eur J Clin Nutr. 2000 Oct;54(10):774-82. doi: 10.1038/sj.ejcn.1601090.
To determine the potential antioxidant effect of rutin (quercetin-3-O-beta-rutinoside) supplementation.
A 6-week randomized single-blind placebo controlled trial was conducted; 500 mg rutin supplement was compared to an equivalent amount of glucose placebo. In addition, a pharmacokinetic study was carried out.
The Rowett Research Institute, Aberdeen, UK.
Eighteen healthy non-obese normocholesterolaemic female volunteers in the age range 18-48 y.
Plasma flavonoids, ascorbic acid, tocopherols and carotenoids, plasma antioxidant capacity, lymphocyte DNA damage, blood chemistry and haematology, liver function tests, urinary malondialdehyde, 8-hydroxy-2-deoxyguanosine and 8-iso-prostaglandin F2alpha.
Eighteen volunteers completed the trial. Rutin supplementation did not induce any adverse changes in blood chemistry or indices of liver function. Plasma flavonoids were significantly elevated in the rutin-supplemented group. Endogenous oxidation of pyrimidines was significantly decreased in both rutin- and placebo-treated volunteers. There was no significant change in the level of urinary 8-hydroxy-2'-deoxyguanosine or urinary malondialdehyde in either group. A linear correlation was observed between urinary malondialdehyde and urinary 8-iso-prostaglandin F2alpha (R = 0.54, P<0.01).
Six weeks' rutin supplementation significantly elevated the levels of three plasma flavonoids (quercetin. kaempferol and isorhamnetin) but there was no significant change in plasma antioxidant status. The decrease in the level of endogenous base oxidation in lymphocyte DNA seen in both the placebo- and rutin-supplemented subjects may reflect seasonal changes in other dietary antioxidants.
确定补充芦丁（槲皮素 - 3 - O - β - 芸香糖苷）的潜在抗氧化作用。
进行了一项为期6周的随机单盲安慰剂对照试验；将500毫克芦丁补充剂与等量的葡萄糖安慰剂进行比较。此外，还开展了一项药代动力学研究。
英国阿伯丁的罗威特研究所。
18名年龄在18 - 48岁之间的健康非肥胖、血脂正常的女性志愿者。
血浆类黄酮、抗坏血酸、生育酚和类胡萝卜素、血浆抗氧化能力、淋巴细胞DNA损伤、血液化学和血液学指标、肝功能测试、尿丙二醛、8 - 羟基 - 2 - 脱氧鸟苷和8 - 异前列腺素F2α。
18名志愿者完成了试验。补充芦丁未引起血液化学或肝功能指标的任何不良变化。补充芦丁组的血浆类黄酮显著升高。芦丁治疗组和安慰剂治疗组的志愿者嘧啶内源性氧化均显著降低。两组尿8 - 羟基 - 2'- 脱氧鸟苷或尿丙二醛水平均无显著变化。观察到尿丙二醛与尿8 - 异前列腺素F2α之间存在线性相关性（R = 0.54，P<0.01）。
补充6周芦丁可显著提高三种血浆类黄酮（槲皮素、山奈酚和异鼠李素）的水平，但血浆抗氧化状态无显著变化。安慰剂组和补充芦丁组受试者淋巴细胞DNA中内源性碱基氧化水平的降低可能反映了其他膳食抗氧化剂的季节性变化。
