Royer A, Beguin S, Sochor H, Communal P Y
Groupement Interrégional de Recherche sur les Produits agropharmaceutiques, Angers Technopole, 8 rue H. Becquerel, 49070 Beaucouzé, France.
J Agric Food Chem. 2000 Nov;48(11):5184-9. doi: 10.1021/jf000281u.
An analytical method for the determination of glufosinate ammonium and its principal metabolites, AE F064619 and AE F061517, in water of two different hardnesses (5 and 30 DH, French hardness) has been developed and validated. Samples were spiked at different levels (0. 05 and 0.5 microgram/L) and were purified by column chromatography on ion-exchange resins. After derivatization with glacial acetic acid and trimethylarthoacetate mixture, the derivatives were quantified by using capillary gas chromatography with an ion-trap tandem mass spectrometric detector. Analytical conditions for MS/MS detection were optimized, and the quantification was carried out on the areas of the most representative ions. The limit of quantification was validated at 0.05 microgram/L for each compound. The mean recovery value and the relative standard deviation (n = 20) were 92.0% and 17. 8% for glufosinate ammonium, 90.2% and 15.8% for AE F064619, and 89. 7% and 12.7% for AE F061517.
已开发并验证了一种用于测定两种不同硬度（5和30 DH，法国硬度）水中草铵膦铵盐及其主要代谢物AE F064619和AE F061517的分析方法。样品在不同水平（0.05和0.5微克/升）下加标，并通过离子交换树脂柱色谱法进行纯化。用冰醋酸和三甲氧基乙酸混合物衍生化后，使用带离子阱串联质谱检测器的毛细管气相色谱法对衍生物进行定量。优化了MS/MS检测的分析条件，并根据最具代表性离子的面积进行定量。每种化合物的定量限均验证为0.05微克/升。草铵膦铵盐的平均回收率和相对标准偏差（n = 20）分别为92.0%和17.8%，AE F064619为90.2%和15.8%，AE F061517为89.7%和12.7%。
