Stander M A, Bornscheuer U T, Henke E, Steyn P S
Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
J Agric Food Chem. 2000 Nov;48(11):5736-9. doi: 10.1021/jf000413j.
Ochratoxin A (OTA) is a nephrotoxic and carcinogenic mycotoxin. The toxin is a common contaminant of various foods and feeds and poses a serious threat to the health of both humans and animals. A number of commercial hydrolases were screened for the ability to degrade OTA to nontoxic compounds. A crude lipase from Aspergillus niger (Amano A) proved to substantially hydrolyze OTA to the nontoxic OTalpha and phenylalanine, as confirmed by HPLC with fluorescence detection. The enzyme was purified by anion exchange chromatography to homogeneity. Activity staining of the purified enzyme with alpha-naphthyl acetate/Fast Red revealed only one band exhibiting hydrolytic activity. The specific activity of the purified enzyme toward OTA was 2.32 units/mg.
赭曲霉毒素A（OTA）是一种具有肾毒性和致癌性的霉菌毒素。该毒素是各种食品和饲料中的常见污染物，对人类和动物的健康构成严重威胁。对多种商业水解酶进行了筛选，以评估其将OTA降解为无毒化合物的能力。经高效液相色谱荧光检测证实，黑曲霉粗脂肪酶（天野A）可将OTA大量水解为无毒的OTα和苯丙氨酸。通过阴离子交换色谱法将该酶纯化至同质。用α-萘乙酸/固红对纯化后的酶进行活性染色，仅显示出一条具有水解活性的条带。纯化后的酶对OTA的比活性为2.32单位/毫克。
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