Hathout Y, Ho Y P, Ryzhov V, Demirev P, Fenselau C
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.
J Nat Prod. 2000 Nov;63(11):1492-6. doi: 10.1021/np000169q.
A novel class of lipopeptides was isolated from Bacillus thuringiensis kurstaki HD-1. Four compounds (1-4) were separated by high-performance liquid chromatography and their primary structures determined using a combination of chemical reactions and mass spectrometry. The four lipopeptides were found to have the same amino acid sequence, Thr-Gly-Ala-Ser-His-Gln-Gln, but different fatty acids. The fatty acyl chain is linked to the N-terminal amino acid residue via an amide bond. Each lipopeptide has a lactone linkage between the carboxyl terminal amino acid and the hydroxyl group in the side chain of the serine residue. Antifungal activity was demonstrated against Stachybotrys charatum.
从苏云金芽孢杆菌库斯塔克亚种HD-1中分离出了一类新型脂肽。通过高效液相色谱法分离出四种化合物（1-4），并结合化学反应和质谱法确定了它们的一级结构。发现这四种脂肽具有相同的氨基酸序列，即苏氨酸-甘氨酸-丙氨酸-丝氨酸-组氨酸-谷氨酰胺-谷氨酰胺，但脂肪酸不同。脂肪酰链通过酰胺键与N端氨基酸残基相连。每个脂肽在羧基末端氨基酸和丝氨酸残基侧链中的羟基之间具有内酯键。已证明对炭疽葡萄穗霉具有抗真菌活性。
