Hanf G, Schierhorn K, Brunnée T, Noga O, Verges D, Kunkel G
Charité Virchow-Klinikum, Humboldt-University, Allergy and Asthma Clinic, Berlin, Germany.
Inflamm Res. 2000 Oct;49(10):520-3. doi: 10.1007/s000110050625.
There is evidence that substance P (SP) is involved in events related to allergic and nonallergic rhinitis. Furthermore, some effects of SP seem to be greater in subjects suffering from allergic rhinitis than in nonallergic subjects. To investigate if these effects may be partly mediated by histamine release (HR) we studied the influence of SP on HR from nasal mucosa of subjects with and without allergic rhinitis using an in vitro organ culture system.
Nasal mucosa of the inferior turbinate was obtained from ten patients suffering from allergic rhinitis and eighteen non-allergic subjects receiving surgical therapy for nasal obstruction.
Tissue samples of nasal mucosa were stimulated with 10(-5) M SP or with 10(-5) M Ca-ionophore A23187 for 120 minutes, and the histamine content was determined in the culture supernatant.
Both SP and Ca-ionophore A23187, caused a significantly higher HR from the samples of the non-allergic group (p < 0.01) compared to baseline controls (spontaneous release). The same effect was seen in the allergic group (p < 0.01 and p = 0.036). Comparing the increase in HR from allergic and non-allergic mucosa, in allergics the HR stimulated by SP was significantly higher (p = 0.031), whereas Ca-ionophore A23187 did not show this effect.
These findings suggest a role of SP in inducing release of histamine from human nasal mucosa, thereby influencing physiologic and pathophysiologic nasal conditions, especially in allergic inflammatory processes.
有证据表明P物质（SP）参与了与变应性和非变应性鼻炎相关的事件。此外，SP对变应性鼻炎患者的某些作用似乎比对非变应性患者的作用更大。为了研究这些作用是否可能部分由组胺释放（HR）介导，我们使用体外器官培养系统研究了SP对有或无变应性鼻炎患者鼻黏膜HR的影响。
从10例变应性鼻炎患者和18例因鼻塞接受手术治疗的非变应性受试者获取下鼻甲鼻黏膜。
用10⁻⁵M SP或10⁻⁵M钙离子载体A23187刺激鼻黏膜组织样本120分钟，然后测定培养上清液中的组胺含量。
与基线对照（自发释放）相比，SP和钙离子载体A23187均使非变应性组样本的HR显著升高（p<0.01）。变应性组也观察到相同的效应（p<0.01和p = 0.036）。比较变应性和非变应性鼻黏膜HR的增加情况，变应性鼻炎患者中SP刺激的HR显著更高（p = 0.031），而钙离子载体A23187未显示出这种效应。
这些发现提示SP在诱导人鼻黏膜组胺释放中起作用，从而影响生理和病理生理状态下的鼻部情况，尤其是在变应性炎症过程中。
