Tanegashima K, Yokota C, Takahashi S, Asashima M
Department of Life Sciences, University of Tokyo, 3-8-1, Komaba, Meguro-ku, 153-8902, Tokyo, Japan.
Mech Dev. 2000 Dec;99(1-2):3-14. doi: 10.1016/s0925-4773(00)00465-2.
In a screening for activin-responsive genes, we isolated a Xenopus lefty/antivin-related gene, called Xantivin (Xatv). In the animal cap assay, the expression of Xatv was induced by activin signaling, and in the embryo, by nodal-related genes. Overexpression of Xatv in the marginal zone caused suppression of mesoderm formation and gastrulation defects, and inhibited the secondary axis formation induced by Xnr1 and Xactivin, suggesting that Xatv acted as a feedback inhibitor of activin signaling. However, in the animal cap, Xatv failed to antagonize Xnr1 and Xactivin. This result suggested that Xatv has different responses in the marginal zone and in the animal region, and antagonizes to a higher degree activin signaling in the marginal zone.
在一项激活素反应基因的筛选中，我们分离出了一个与非洲爪蟾Lefty/抗激活素相关的基因，称为非洲爪蟾抗激活素（Xatv）。在动物帽实验中，Xatv的表达由激活素信号诱导，而在胚胎中则由与节点相关的基因诱导。在边缘区过表达Xatv会导致中胚层形成受到抑制和原肠胚形成缺陷，并抑制由Xnr1和X激活素诱导的次级轴形成，这表明Xatv作为激活素信号的反馈抑制剂发挥作用。然而，在动物帽中，Xatv未能拮抗Xnr1和X激活素。这一结果表明，Xatv在边缘区和动物区域有不同的反应，并且在边缘区对激活素信号的拮抗作用更强。
