Le Guen S, Prost C, Demaimay M
Ecole Nationale d'Ingénieurs des Techniques et Industries Agro-Alimentaires, Laboratoire de Biochimie Industrielle et Alimentaire, BP 82225, Nantes, France.
J Chromatogr A. 2000 Oct 27;896(1-2):361-71. doi: 10.1016/s0021-9673(00)00729-9.
Gas chromatography-olfactometry consists of sniffing the effluent of a gas chromatograph and leads to the direct determination of potent odorants in food. GC-olfactometry and GC-MS were applied in order to identify volatile compounds, and to characterize potent odorants of cooked wild mussels and bouchot mussels. Eighty-five volatiles were identified by GC-MS, among those the majority were identified for the first time in mussels. Using GC-olfactometry, the main contributors of cooked mussels aroma were characterized. Of the 85 volatiles identified in the flavor, only 33 were odor-active and contribute to the overall aroma of mussels. Dimethyl disulfide (sulfury odor) was the odorant the most differently perceived between the two extracts and seems to be characteristic of wild mussels. Combined GC-MS and GC-olfactometry made it possible to point out odorants which actually contribute to the aroma of cooked mussels and those which showed typical dependence on the origin of mussels.
气相色谱 - 嗅觉测量法是通过嗅闻气相色谱的流出物来直接测定食品中的强效气味物质。应用气相色谱 - 嗅觉测量法和气相色谱 - 质谱联用技术来鉴定挥发性化合物,并表征野生贻贝和垂养贻贝烹饪后的强效气味物质。通过气相色谱 - 质谱联用技术鉴定出了85种挥发性物质,其中大多数是首次在贻贝中鉴定出来的。利用气相色谱 - 嗅觉测量法,对烹饪贻贝香气的主要成分进行了表征。在风味中鉴定出的85种挥发性物质中,只有33种具有气味活性,并对贻贝的整体香气有贡献。二甲基二硫醚(硫磺气味)是两种提取物中气味感知差异最大的气味物质,似乎是野生贻贝的特征性物质。气相色谱 - 质谱联用技术和气相色谱 - 嗅觉测量法相结合,能够指出实际对烹饪贻贝香气有贡献的气味物质,以及那些表现出对贻贝来源有典型依赖性的气味物质。