Vinogradov E, Cedzynski M, Rozalski A, Ziolkowski A, Swierzko A
Institute for Biological Sciences, National Research Council, Ottawa, Ont., Canada.
Carbohydr Res. 2000 Oct 6;328(4):533-8. doi: 10.1016/s0008-6215(00)00134-8.
The following structure of the lipid A-core region of the lipopolysaccharide (LPS) from Proteus vulgaris serotype O25 was determined by using NMR and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of LPS, of the products of alkaline deacylation of the LPS, and of the products of LPS deamination: [structure: see text] Terminal residues of beta-GlcNAc and beta-Kdo (indicated by bold italics) are present alternatively in approximately 3:2 amount, leaving no unsubstituted beta-Gal. All sugars are in the pyranose form, alpha-Hep is the residue of L-glycero-alpha-D-manno-Hep, alpha-DDHep is the residue of D-glycero-alpha-D-manno-Hep.
通过对普通变形杆菌O25血清型脂多糖（LPS）的核心寡糖进行核磁共振（NMR）和化学分析，确定了LPS脂质A核心区域的以下结构。该核心寡糖通过LPS的温和酸水解、LPS的碱脱酰产物以及LPS脱氨产物获得：[结构：见原文]β - GlcNAc和β - Kdo的末端残基（用粗斜体表示）交替存在，比例约为3:2，没有未取代的β - Gal。所有糖类均为吡喃糖形式，α - Hep是L - 甘油 - α - D - 甘露 - 庚糖的残基，α - DDHep是D - 甘油 - α - D - 甘露 - 庚糖的残基。
