Banks R E, Dunn M J, Hochstrasser D F, Sanchez J C, Blackstock W, Pappin D J, Selby P J
ICRF Cancer Medicine Research Unit, St James's University Hospital, Leeds, UK.
Lancet. 2000 Nov 18;356(9243):1749-56. doi: 10.1016/S0140-6736(00)03214-1.
Proteomics-based approaches, which examine the expressed proteins of a tissue or cell type, complement the genome initiatives and are increasingly being used to address biomedical questions. Proteins are the main functional output, and the genetic code cannot always indicate which proteins are expressed, in what quantity, and in what form. For example, post-translational modifications of proteins, such as phosphorylation or glycosylation, are very important in determining protein function. Similarly, the effects of environmental factors or multigenic processes such as ageing or disease cannot be assessed simply by examination of the genome alone. This review describes the underlying technology and illustrates several areas of biomedical research, ranging from pathogenesis of neurological disorders to drug and vaccine design, in which potential clinical applications are being explored.
基于蛋白质组学的方法用于检测组织或细胞类型中表达的蛋白质，它是对基因组计划的补充，并且越来越多地被用于解决生物医学问题。蛋白质是主要的功能输出物，而遗传密码并不总能指示哪些蛋白质被表达、表达量以及表达形式。例如，蛋白质的翻译后修饰，如磷酸化或糖基化，在确定蛋白质功能方面非常重要。同样，环境因素或多基因过程（如衰老或疾病）的影响不能仅通过检查基因组来简单评估。本综述描述了相关基础技术，并举例说明了生物医学研究的几个领域，从神经疾病的发病机制到药物和疫苗设计，其中正在探索潜在的临床应用。
