Hirata S, Shoda T, Kato J, Hoshi K
Department of Obstetrics and Gynecology, Yamanashi Medical University, Nakakoma, Yamanashi, Japan.
Oncology. 2000;59 Suppl 1:39-44. doi: 10.1159/000055286.
A novel isoform (termed isoform S) of the progesterone receptor (PR) cDNA (PR isoform S cDNA) which consists of a previously unidentified 5' sequence and exons 4-8 of the intracellular PR gene has been cloned from the human testicular cDNA library. The 5' sequence of the message was confirmed to be derived from a novel exon (termed exon S) by genomic cloning. The expression level of the PR isoform S mRNA was higher in the spermatozoon than in the uterine endometrium with a lower expression level of the PR isoforms B and A mRNAs in the spermatozoon than in the endometrium. These results implied that the PR isoform S which was possibly translated from the PR isoform S mRNA in the spermatozoon might be related to the cell surface membrane PR. Moreover, the PR isoform S in the uterine endometrium might play some physiological and/or pathogenic roles.
从人睾丸cDNA文库中克隆出一种新型孕酮受体（PR）cDNA同工型（称为同工型S）（PR同工型S cDNA），其由细胞内PR基因先前未鉴定的5'序列和外显子4-8组成。通过基因组克隆证实该信使RNA的5'序列来自一个新的外显子（称为外显子S）。PR同工型S mRNA在精子中的表达水平高于子宫内膜，而PR同工型B和A mRNA在精子中的表达水平低于子宫内膜。这些结果表明，精子中可能由PR同工型S mRNA翻译而来的PR同工型S可能与细胞表面膜PR有关。此外，子宫内膜中的PR同工型S可能发挥一些生理和/或致病作用。
