Benech-Kieffer F, Wegrich P, Schwarzenbach R, Klecak G, Weber T, Leclaire J, Schaefer H
Life Sciences, Advanced Research Laboratories, L'Oréal, Aulnay-sous-Bois, France.
Skin Pharmacol Appl Skin Physiol. 2000 Nov-Dec;13(6):324-35. doi: 10.1159/000029940.
Appropriate evaluation of sunscreens is required to provide better knowledge of their safety and efficacy. One of the most important elements of this evaluation is the assessment of percutaneous absorption. In vitro methods are largely used for such assessments, and the accuracy of the measurements generated with these methods depends on the use of a proper methodology. This study was designed to evaluate an in vitro protocol for investigating the percutaneous absorption of two sunscreens under standardized experimental conditions. Octyl methoxycinnamate and benzophenone 4 were each incorporated in a typical oil-in-water emulsion and tested separately. Salicylic acid was tested as a reference compound. In vitro percutaneous absorption was evaluated using two species, the pig and human, and two models, full-thickness and split-thickness skin. The reproducibility of study results was evaluated by comparing the data generated by two industrial laboratories, L'Oréal and Hoffmann-La Roche. The correlation of quantitative data between pig skin and human skin was very good, and the split-thickness skin model seemed to be more appropriate for measuring the absorption of sunscreens. Results obtained for salicylic acid demonstrated the relevance of the protocol in terms of prediction of in vivo percutaneous absorption. Finally, the comparison of pig skin data between the two laboratories demonstrated a good correlation and underlined the need for a standardized in vitro procedure.
为了更好地了解防晒霜的安全性和有效性，需要对其进行适当评估。这种评估最重要的要素之一是经皮吸收的评估。体外方法在很大程度上用于此类评估，并且这些方法所产生测量结果的准确性取决于适当方法的使用。本研究旨在评估一种体外实验方案，用于在标准化实验条件下研究两种防晒霜的经皮吸收。桂皮酸盐和二苯甲酰甲烷分别加入典型的水包油乳液中并分别进行测试。水杨酸作为参考化合物进行测试。使用猪和人两个物种以及全层和分层皮肤两种模型评估体外经皮吸收。通过比较欧莱雅和霍夫曼 - 罗氏两个工业实验室生成的数据来评估研究结果的可重复性。猪皮和人皮之间定量数据的相关性非常好，分层皮肤模型似乎更适合测量防晒霜的吸收。水杨酸获得的结果证明了该实验方案在预测体内经皮吸收方面的相关性。最后，两个实验室之间猪皮数据的比较显示出良好的相关性，并强调了标准化体外程序的必要性。
