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Appl Environ Microbiol. 2000 Dec;66(12):5134-40. doi: 10.1128/AEM.66.12.5134-5140.2000.
2
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Engineering of the substrate-binding region of the subtilisin-like, cell-envelope proteinase of Lactococcus lactis.乳酸乳球菌类枯草杆菌蛋白酶样细胞包膜蛋白酶底物结合区域的工程改造
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Peptides inhibitory to endopeptidase and aminopeptidase from Lactococcus lactis ssp. lactis MG1363, released from bovine beta-casein by chymosin, trypsin or chymotrypsin.对来自乳酸乳球菌乳亚种MG1363的内肽酶和氨肽酶具有抑制作用的肽，这些肽由凝乳酶、胰蛋白酶或胰凝乳蛋白酶从牛β-酪蛋白中释放出来。
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本文引用的文献

1
Improved medium for lactic streptococci and their bacteriophages.用于乳酸链球菌及其噬菌体的改良培养基。
Appl Microbiol. 1975 Jun;29(6):807-13. doi: 10.1128/am.29.6.807-813.1975.
2
Altered specificity of lactococcal proteinase p(i) (lactocepin I) in humectant systems reflecting the water activity and salt content of cheddar cheese.在反映切达干酪水分活度和盐含量的保湿系统中，乳球菌蛋白酶 p(i)（乳凝素 I）的特异性发生改变。
Appl Environ Microbiol. 1998 Feb;64(2):588-93. doi: 10.1128/AEM.64.2.588-593.1998.
3
Minimal Requirements for Exponential Growth of Lactococcus lactis.乳球菌指数生长的最小要求。
Appl Environ Microbiol. 1993 Dec;59(12):4363-6. doi: 10.1128/aem.59.12.4363-4366.1993.
4
Autoproteolysis of the Extracellular Serine Proteinase of Lactococcus lactis subsp. cremoris Wg2.乳球菌乳脂亚种 Wg2 的细胞外丝氨酸蛋白酶的自身水解。
Appl Environ Microbiol. 1991 Sep;57(9):2586-90. doi: 10.1128/aem.57.9.2586-2590.1991.
5
Mechanism of Proteinase Release from Lactococcus lactis subsp. cremoris Wg2.乳球菌乳亚种 Wg2 蛋白酶释放机制。
Appl Environ Microbiol. 1989 Dec;55(12):3101-6. doi: 10.1128/aem.55.12.3101-3106.1989.
6
Monoclonal Antibodies to the Cell-Wall-Associated Proteinase of Lactococcus lactis subsp. cremoris Wg2.乳球菌细胞壁相关蛋白酶单克隆抗体。
Appl Environ Microbiol. 1988 Sep;54(9):2250-6. doi: 10.1128/aem.54.9.2250-2256.1988.
7
Comparative Study of Action of Cell Wall Proteinases from Various Strains of Streptococcus cremoris on Bovine alpha(s1)-, beta-, and kappa-Casein.不同来源的乳链菌细胞壁蛋白酶对牛α(s1)-、β-和 κ-酪蛋白作用的比较研究。
Appl Environ Microbiol. 1986 Nov;52(5):1162-6. doi: 10.1128/aem.52.5.1162-1166.1986.
8
Deletion of various carboxy-terminal domains of Lactococcus lactis SK11 proteinase: effects on activity, specificity, and stability of the truncated enzyme.乳酸乳球菌SK11蛋白酶各种羧基末端结构域的缺失：对截短型酶的活性、特异性和稳定性的影响。
Appl Environ Microbiol. 2000 Jul;66(7):2859-65. doi: 10.1128/AEM.66.7.2859-2865.2000.
9
Accumulation of casein-derived peptides during growth of proteinase-positive strains of Lactococcus lactis in milk: their contribution to subsequent bacterial growth is impaired by their internal transport.乳酸乳球菌蛋白酶阳性菌株在牛奶中生长期间酪蛋白衍生肽的积累：其内部转运削弱了它们对后续细菌生长的促进作用。
J Dairy Res. 2000 May;67(2):233-40. doi: 10.1017/s0022029900004192.
10
HtrA is the unique surface housekeeping protease in Lactococcus lactis and is required for natural protein processing.HtrA是乳酸乳球菌中唯一的表面管家蛋白酶，是天然蛋白质加工所必需的。
Mol Microbiol. 2000 Mar;35(5):1042-51. doi: 10.1046/j.1365-2958.2000.01757.x.

乳酸乳球菌乳链菌蛋白酶III的自催化作用影响其对β-酪蛋白的特异性。

The autoproteolysis of Lactococcus lactis lactocepin III affects its specificity towards beta-casein.

作者信息

Flambard B, Juillard V

机构信息

Unité de Recherches Laitières et Génétique Appliquée, Institut National de la Recherche Agronomique, F-78350 Jouy-en-Josas, France.

出版信息

Appl Environ Microbiol. 2000 Dec;66(12):5134-40. doi: 10.1128/AEM.66.12.5134-5140.2000.

DOI:10.1128/AEM.66.12.5134-5140.2000
PMID:11097880
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC92434/
Abstract

The effect of autoproteolysis of Lactococcus lactis lactocepin III on its specificity towards beta-casein was investigated. beta-Casein degradation was performed by using either an autolysin-defective derivative of L. lactis MG1363 carrying the proteinase genes of L. lactis SK11, which was unable to transport oligopeptides, or autoproteolyzed enzyme purified from L. lactis SK11. Comparison of the peptide pools by high-performance liquid chromatography analysis revealed significant differences. To analyze these differences in more detail, the peptides released by the cell-anchored proteinase were identified by on-line coupling of liquid chromatography to mass spectrometry. More than 100 oligopeptides were released from beta-casein by the cell-anchored proteinase. Analysis of the cleavage sites indicated that the specificity of peptide bond cleavage by the cell-anchored proteinase differed significantly from that of the autoproteolyzed enzyme.

摘要

研究了乳酸乳球菌乳酸蛋白酶III的自催化作用对其β-酪蛋白特异性的影响。使用携带乳酸乳球菌SK11蛋白酶基因的乳酸乳球菌MG1363自溶素缺陷衍生物（其无法转运寡肽）或从乳酸乳球菌SK11纯化的自催化酶进行β-酪蛋白降解。通过高效液相色谱分析对肽库进行比较，结果显示存在显著差异。为了更详细地分析这些差异，通过液相色谱与质谱联用在线鉴定细胞锚定蛋白酶释放的肽。细胞锚定蛋白酶从β-酪蛋白中释放出100多种寡肽。对切割位点的分析表明，细胞锚定蛋白酶的肽键切割特异性与自催化酶的肽键切割特异性有显著差异。