用于生态研究的rRNA直接提取与纯化
Direct extraction and purification of rRNA for ecological studies.
作者信息
Moran M A, Torsvik V L, Torsvik T, Hodson R E
机构信息
Department of Microbiology, University of Georgia, Athens 30602-2605.
出版信息
Appl Environ Microbiol. 1993 Mar;59(3):915-8. doi: 10.1128/aem.59.3.915-918.1993.
Abstract
Microgram quantities of rRNA were recovered from natural microbial communities in sediment, soil, and water with a lysozyme-hot phenol direct extraction method. Gel filtration with Sephadex G-75 spun columns readily removed humic-like contaminants without any measurable loss of rRNA and rendered RNA extracts of sufficient quality for molecular procedures.
摘要
采用溶菌酶 - 热酚直接提取法，从沉积物、土壤和水中的天然微生物群落中回收了微克量的rRNA。使用Sephadex G - 75旋转柱进行凝胶过滤，能轻松去除类腐殖质污染物，且rRNA没有任何可测量的损失，得到了质量足以用于分子操作的RNA提取物。
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