Ramalho-Santos J, Sutovsky P, Simerly C, Oko R, Wessel G M, Hewitson L, Schatten G
Oregon Regional Primate Research Center, Division of Reproductive Sciences, Beaverton, Oregon 97006, USA.
Hum Reprod. 2000 Dec;15(12):2610-20. doi: 10.1093/humrep/15.12.2610.
We have dissected the initial stages of fertilization by intracytoplasmic sperm injection of single spermatozoa into prime oocytes from fertile rhesus monkeys (Macaca mulatta). DNA decondensation was delayed at the apical portion of the sperm head. It is possible that this asynchronous male DNA decondensation could be related to the persistence of the sperm acrosome and perinuclear theca after injection. However, incomplete male pronuclear formation did not prevent sperm aster formation, microtubule nucleation and pronuclear apposition. In contrast, DNA synthesis was delayed in both pronuclei until the sperm chromatin fully decondensed, indicating that male pronuclear formation constitutes an important checkpoint during the first embryonic cell cycle.
我们通过将单个精子胞浆内注射到来自可育恒河猴（猕猴）的优质卵母细胞中，剖析了受精的初始阶段。精子头部顶端部分的DNA解聚延迟。这种异步的雄性DNA解聚可能与注射后精子顶体和核周膜的持续存在有关。然而，雄性原核形成不完全并未阻止精子星体形成、微管成核和原核并置。相比之下，两个原核中的DNA合成均延迟，直到精子染色质完全解聚，这表明雄性原核形成是第一个胚胎细胞周期中的一个重要检查点。
