Quarleri J F, Robertson B H, Mathet V L, Feld M, Espínola L, Requeijo M P, Mandó O, Carballal G, Oubiña J R
Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
J Clin Microbiol. 2000 Dec;38(12):4560-8. doi: 10.1128/JCM.38.12.4560-4568.2000.
Typing of hepatitis C virus (HCV) isolates from Argentine patients was performed by using different methodologies in a population of 243 patients. HCV subtype was assigned based upon restriction fragment length polymorphism (RFLP). HCV RNA genomes obtained from serum samples were classified as belonging to clade 1 (53.5%), 2 (23. 0%), or 3 (8.6%); 14.8% of samples showed HCV mixed infections, more frequently implying different subtypes within the same clade. In addition to RFLP typing, phylogenetic relatedness among sequences from both 5' untranslated region (n = 50) and nonstructural 5B coding region (n = 15) was established.
在243名阿根廷患者群体中,采用不同方法对丙型肝炎病毒(HCV)分离株进行分型。基于限制性片段长度多态性(RFLP)确定HCV亚型。从血清样本中获得的HCV RNA基因组被分类为属于1型(53.5%)、2型(23.0%)或3型(8.6%);14.8%的样本显示HCV混合感染,更常见的是同一分支内不同亚型的混合感染。除了RFLP分型外,还确定了来自5'非翻译区(n = 50)和非结构5B编码区(n = 15)序列之间的系统发育相关性。