阿根廷患者丙型肝炎病毒分离株的基因组和系统发育分析：一项为期六年的回顾性研究。
Genomic and phylogenetic analysis of hepatitis C virus isolates from argentine patients: a six-year retrospective study.
作者信息
Quarleri J F, Robertson B H, Mathet V L, Feld M, Espínola L, Requeijo M P, Mandó O, Carballal G, Oubiña J R
机构信息
Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
出版信息
J Clin Microbiol. 2000 Dec;38(12):4560-8. doi: 10.1128/JCM.38.12.4560-4568.2000.
Abstract
Typing of hepatitis C virus (HCV) isolates from Argentine patients was performed by using different methodologies in a population of 243 patients. HCV subtype was assigned based upon restriction fragment length polymorphism (RFLP). HCV RNA genomes obtained from serum samples were classified as belonging to clade 1 (53.5%), 2 (23. 0%), or 3 (8.6%); 14.8% of samples showed HCV mixed infections, more frequently implying different subtypes within the same clade. In addition to RFLP typing, phylogenetic relatedness among sequences from both 5' untranslated region (n = 50) and nonstructural 5B coding region (n = 15) was established.
摘要
在243名阿根廷患者群体中，采用不同方法对丙型肝炎病毒（HCV）分离株进行分型。基于限制性片段长度多态性（RFLP）确定HCV亚型。从血清样本中获得的HCV RNA基因组被分类为属于1型（53.5%）、2型（23.0%）或3型（8.6%）；14.8%的样本显示HCV混合感染，更常见的是同一分支内不同亚型的混合感染。除了RFLP分型外，还确定了来自5'非翻译区（n = 50）和非结构5B编码区（n = 15）序列之间的系统发育相关性。
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