Jørgensen K H, Hansen A K, Buschard K
Bartholin Instituttet, Kommunehospitalet, DK-1399, Copenhagen, Denmark.
Diabetes Res Clin Pract. 2000 Dec;50(3):161-7. doi: 10.1016/s0168-8227(00)00196-0.
Human NPH U100 (100 IU/ml), given once daily, is often absorbed too fast to cover the basal insulin demand throughout 24 h. The aim of the present study was to examine whether the absorption of human insulin suspensions would be delayed by increasing the insulin concentration from U100 to U500. In each experiment 10 IU of corresponding human Ul00 and U500 preparations, labelled with 125I-insulin, were injected subcutaneously and contralaterally in the neck of a pig followed by monitoring residual radioactivity over the injection sites. The time until 75, 50 and 25% residual radioactivity (T(75%), T(50%) and T(25%)) using NPH U500 was compared with NPH U100 in 14 experiments: T(75%): 5.0+/-0.5 (mean+/-SEM) vs 3.8+/-0.3 h (P=0.007, paired t-test), T(50%): 12. 2+/-0.9 vs 9.0+/-0.6 h (P=0.003) and T(25%). 24.2+/-1.2 vs 17.9+/-1. 2 h (P=0.001). The corresponding values for semilente U500 compared with semilente U100 in eight experiments were: T(75%): 2.8+/-0.4 vs 1.6+/-0.2 h (P=0.02), T(50%): 5.6+/-0.6 vs 3.4+/-0.3 h (P=0.01) and T(25%): 10.9+/-1.1 vs 7.2+/-0.7 h (P=0.009). Thus, the absorption of a given dose of human NPH or human semilente insulin in pigs is substantially delayed by changing the insulin concentration from Ul00 to U500. Human NPH U500 appears to be more appropriate than human NPH U100 for injection once daily in basal insulin therapy.
人中性鱼精蛋白锌胰岛素U100（100IU/ml），每日注射一次，其吸收往往过快，无法满足24小时的基础胰岛素需求。本研究的目的是检验将胰岛素浓度从U100提高到U500是否会延迟人胰岛素混悬液的吸收。在每个实验中，将10IU相应的人U100和U500制剂，用125I标记胰岛素，皮下注射到猪颈部的对侧，然后监测注射部位的残留放射性。在14个实验中，比较了使用NPH U500与NPH U100时达到75%、50%和25%残留放射性的时间（T(75%)、T(50%)和T(25%)）：T(75%)：5.0±0.5（平均值±标准误）对3.8±0.3小时（P=0.007，配对t检验），T(50%)：12.2±0.9对9.0±0.6小时（P=0.003），T(25%)：24.2±1.2对17.9±1.2小时（P=0.001）。在8个实验中，比较了半慢胰岛素U500与半慢胰岛素U100的相应值：T(75%)：2.8±0.4对1.6±0.2小时（P=0.02），T(50%)：5.6±0.6对3.4±0.3小时（P=0.01），T(25%)：10.9±1.1对7.2±0.7小时（P=0.009）。因此，通过将胰岛素浓度从U100改为U500，猪体内给定剂量的人中性鱼精蛋白锌胰岛素或人半慢胰岛素的吸收会显著延迟。人中性鱼精蛋白锌胰岛素U500似乎比人中性鱼精蛋白锌胰岛素U100更适合在基础胰岛素治疗中每日注射一次。
