Bar M, Shannon-Lowe C, Geballe A P
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA.
J Infect Dis. 2001 Jan 15;183(2):218-225. doi: 10.1086/317939. Epub 2000 Dec 8.
The variety of clinical manifestations of human cytomegalovirus infection probably results from both viral and host factors. Because genetic markers that span the viral genome are needed to identify such viral factors, polymorphisms at the UL4 gene locus were analyzed. DNA sequence analyses revealed 4 UL4-based genotypes, 2 of which were closely related but distinguishable by an uncommon polymorphism that results in overexpression of gpUL4. Similarities in the spectra of polymorphisms detected in various sets of samples reveal that all UL4 types infect a diversity of organs in different patient groups and in different geographic locales. Simultaneous infection by >1 UL4 type is common in AIDS patients. Data from sequencing analyses and from a rapid and simple UL4 typing assay did not detect linkage between UL4 and glycoprotein B types, which suggests that UL4 genotyping should be useful for studies that attempt to identify cytomegalovirus genes involved in disease pathogenesis.
人类巨细胞病毒感染临床表现的多样性可能是由病毒和宿主因素共同导致的。由于需要跨越病毒基因组的遗传标记来识别此类病毒因素，因此对UL4基因座的多态性进行了分析。DNA序列分析揭示了4种基于UL4的基因型，其中2种密切相关，但可通过一种罕见的多态性加以区分，这种多态性会导致gpUL4的过度表达。在不同样本组中检测到的多态性谱的相似性表明，所有UL4类型均可感染不同患者群体和不同地理位置的多种器官。艾滋病患者中同时感染>1种UL4类型的情况很常见。测序分析数据以及一项快速简便的UL4分型检测未检测到UL4与糖蛋白B类型之间的关联，这表明UL4基因分型对于试图识别参与疾病发病机制的巨细胞病毒基因的研究应是有用的。
