Bullock L P, Bardin C W
Steroids. 1975 Jan;25(1):107-19. doi: 10.1016/s0039-128x(75)80011-0.
The in vivo retention of 3-H-testosterone, dihydrotestosterone (DHT), 3alpha-androstanediol (3alpha-DIOL), 3beta-DIOL, androstenedione, progesterone and cortisol by renal cytoplasm and nuclei of male and female mice was studied. Testosterone was the major androgen isolated from cytoplasm and nuclei following testosterone or androstenedione administration. By contrast, DHT was the major intracellular androgen after DHT, 3alpha- or 3beta-DIOL injection. The uptake of 3-H-testosterone or 3-H-DHT was abolished by excess unlabeled testosterone, DHT or cyproterone acetate. Androgen concentrations in kidney fractions from female mice were similar to those from males. There was no appreciable concentration of the isolated steroids following 3-H-progesterone administration. 3H-cortisol was concentrated in both cytoplasm and nuclei but was not displaced by non-radioactive androgens. These findings suggest that in contrast to prostate, mouse kidney can concentrate both testosterone and DHT. However, since testosterone is the major androgen in blood and since it is not metabolized in kidney, it is the major effector androgen in this organ. Androstenedione is active via conversion to testosterone while DIOLS are androgenic via metabolism to DHT.
研究了雄性和雌性小鼠肾细胞质和细胞核对3 - H - 睾酮、双氢睾酮（DHT）、3α - 雄烷二醇（3α - DIOL）、3β - DIOL、雄烯二酮、孕酮和皮质醇的体内滞留情况。给予睾酮或雄烯二酮后，睾酮是从细胞质和细胞核中分离出的主要雄激素。相比之下，注射DHT、3α - 或3β - DIOL后，DHT是主要的细胞内雄激素。过量未标记的睾酮、DHT或醋酸环丙孕酮可消除对3 - H - 睾酮或3 - H - DHT的摄取。雌性小鼠肾部分中的雄激素浓度与雄性小鼠相似。给予3 - H - 孕酮后，分离出的类固醇没有明显的浓度变化。3H - 皮质醇在细胞质和细胞核中均有浓缩，但不会被非放射性雄激素取代。这些发现表明，与前列腺不同，小鼠肾脏可以浓缩睾酮和DHT。然而，由于睾酮是血液中的主要雄激素，且在肾脏中不被代谢，所以它是该器官中的主要效应雄激素。雄烯二酮通过转化为睾酮发挥作用，而二醇类则通过代谢为DHT产生雄激素作用。
