毛细管区带电泳法测定士的宁和马钱子碱

Determination of strychnine and brucine by capillary zone electrophoresis.

作者信息

Zong Y Y, Che C T

机构信息

Department of Chemistry, Hong Kong University of Science and Technology, Kowloon.

出版信息

Planta Med. 1995 Oct;61(5):456-8. doi: 10.1055/s-2006-958136.

DOI:10.1055/s-2006-958136

PMID:7480208

Abstract

A procedure for quantitative estimation of strychnine and brucine in the extracts of Strychnos nux-vomica seeds by capillary zone electrophoresis (CZE) was developed. The buffer solution used was 10mM phosphate buffer-MeOH (9:1), pH 2.5. The linear calibration range was 0.01-0.15 mg/ml. This method is useful for the qualitative and quantitative determination of strychnine and brucine in plant drug samples, as well as in human plasma.

摘要

建立了一种通过毛细管区带电泳（CZE）定量测定马钱子种子提取物中士的宁和马钱子碱的方法。所用缓冲溶液为10mM磷酸盐缓冲液-甲醇（9:1），pH 2.5。线性校准范围为0.01-0.15mg/ml。该方法可用于植物药样品以及人体血浆中士的宁和马钱子碱的定性和定量测定。