采用场增强堆积的毛细管电泳法快速灵敏地测定士的宁和马钱子碱。

Capillary electrophoresis with field-enhanced stacking for rapid and sensitive determination of strychnine and brucine.

作者信息

Zhang Jiyou, Wang Shufang, Chen Xingguo, Hu Zhide, Ma Xiao

机构信息

Department of Chemistry, Lanzhou University, 730000 Lanzhou, PR China.

出版信息

Anal Bioanal Chem. 2003 May;376(2):210-3. doi: 10.1007/s00216-003-1852-y. Epub 2003 Apr 12.

DOI:10.1007/s00216-003-1852-y

PMID:12692702

Abstract

A new capillary electrophoresis procedure with field-enhanced stacking concentration for the analysis of strychnine and brucine is established. After optimization of the separation and concentration conditions, the two alkaloids can be separated within 5 min and quantified with high sensitivity (The detection limits were 1.0 ng mL(-1) for strychnine and 1.4 ng mL(-1) for brucine). The method was useful for qualitative and quantitative analysis of strychnine and brucine in Strychnos nux-vomica L with recovery of 105.1% for strychnine and 98.4% for brucine.

摘要

建立了一种用于分析士的宁和马钱子碱的具有场增强堆积富集作用的新型毛细管电泳方法。在优化分离和富集条件后，两种生物碱可在5分钟内分离，并能高灵敏度定量（士的宁的检测限为1.0 ng mL⁻¹，马钱子碱的检测限为1.4 ng mL⁻¹）。该方法可用于马钱子中士的宁和马钱子碱的定性和定量分析，士的宁回收率为105.1%，马钱子碱回收率为98.4%。