费氏弧菌中的群体感应：通过丙氨酸扫描诱变分析LuxR DNA结合区域
Quorum sensing in Vibrio fischeri: analysis of the LuxR DNA binding region by alanine-scanning mutagenesis.
作者信息
Egland K A, Greenberg E P
机构信息
Department of Microbiology and Graduate Program in Molecular Biology, University of Iowa, Iowa City, Iowa 52242, USA.
出版信息
J Bacteriol. 2001 Jan;183(1):382-6. doi: 10.1128/JB.183.1.382-386.2001.
Abstract
LuxR is the transcriptional activator for quorum-sensing control of luminescence in Vibrio fischeri. A series of alanine-scanning mutants spanning a predicted helix-turn-helix region in the DNA binding domain of LuxR was constructed, and the activity of each of the LuxR mutant proteins in recombinant Escherichia coli was investigated. The region covered by the mutagenesis spanned residues 190 to 224. About half of the alanine-scanning mutants showed activities similar to that of the wild-type LuxR: at least two were positive-control mutants, four appeared to be defective in DNA binding, and several others were characterized as DNA binding affinity mutants. This analysis, taken together with information about other bacterial transcription factors, provides insights into amino acid residues in LuxR that are involved in DNA binding and transcriptional activation.
摘要
LuxR是费氏弧菌群体感应控制发光的转录激活因子。构建了一系列跨越LuxR DNA结合域中预测的螺旋-转角-螺旋区域的丙氨酸扫描突变体，并研究了每种LuxR突变蛋白在重组大肠杆菌中的活性。诱变覆盖的区域跨度为190至224位氨基酸残基。大约一半的丙氨酸扫描突变体表现出与野生型LuxR相似的活性：至少两个是阳性对照突变体，四个似乎在DNA结合方面存在缺陷，其他几个被表征为DNA结合亲和力突变体。该分析与关于其他细菌转录因子的信息相结合，为LuxR中参与DNA结合和转录激活的氨基酸残基提供了见解。
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本文引用的文献
1
Overproduction and purification of the luxR gene product: Transcriptional activator of the Vibrio fischeri luminescence system.LuxR 基因产物的过度生产和纯化：发光系统的 Vibrio fischeri 的转录激活剂。
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6639-43. doi: 10.1073/pnas.84.19.6639.
2
Conversion of the Vibrio fischeri transcriptional activator, LuxR, to a repressor.费氏弧菌转录激活因子LuxR向阻遏物的转变。
J Bacteriol. 2000 Feb;182(3):805-11. doi: 10.1128/JB.182.3.805-811.2000.
3
Signal-dependent DNA binding and functional domains of the quorum-sensing activator TraR as identified by repressor activity.通过阻遏物活性鉴定的群体感应激活因子TraR的信号依赖性DNA结合和功能结构域
Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9009-14. doi: 10.1073/pnas.96.16.9009.
4
Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells.群体感应调节因子TraR与自诱导物的结合在体外增加了对靶启动子的亲和力，并降低了全细胞中TraR的周转速率。
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4832-7. doi: 10.1073/pnas.96.9.4832.
5
Quorum sensing in Vibrio fischeri: elements of the luxl promoter.费氏弧菌中的群体感应：luxI启动子元件
Mol Microbiol. 1999 Feb;31(4):1197-204. doi: 10.1046/j.1365-2958.1999.01261.x.
6
Self perception in bacteria: quorum sensing with acylated homoserine lactones.细菌中的自我感知：酰化高丝氨酸内酯群体感应
Curr Opin Microbiol. 1998 Apr;1(2):183-9. doi: 10.1016/s1369-5274(98)80009-x.
7
Integration of the quorum-sensing system in the regulatory networks controlling virulence factor synthesis in Erwinia chrysanthemi.群体感应系统整合于控制菊欧文氏菌毒力因子合成的调控网络中。
Mol Microbiol. 1998 Sep;29(6):1407-18. doi: 10.1046/j.1365-2958.1998.01023.x.
8
Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa.铜绿假单胞菌中las和rhl群体感应的调控
J Bacteriol. 1997 May;179(10):3127-32. doi: 10.1128/jb.179.10.3127-3132.1997.
9
Critical base pairs and amino acid residues for protein-DNA interaction between the TyrR protein and tyrP operator of Escherichia coli.大肠杆菌TyrR蛋白与tyrP操纵基因之间蛋白质-DNA相互作用的关键碱基对和氨基酸残基
J Bacteriol. 1997 Feb;179(4):1051-8. doi: 10.1128/jb.179.4.1051-1058.1997.
10
Quorum sensing in Vibrio fischeri: essential elements for activation of the luminescence genes.费氏弧菌中的群体感应：激活发光基因的关键要素。
J Bacteriol. 1997 Jan;179(2):557-62. doi: 10.1128/jb.179.2.557-562.1997.
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