White J A, Todd J, Newman T, Focks N, Girke T, de Ilárduya O M, Jaworski J G, Ohlrogge J B, Benning C
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA.
Plant Physiol. 2000 Dec;124(4):1582-94. doi: 10.1104/pp.124.4.1582.
Large-scale single-pass sequencing of cDNAs from different plants has provided an extensive reservoir for the cloning of genes, the evaluation of tissue-specific gene expression, markers for map-based cloning, and the annotation of genomic sequences. Although as of January 2000 GenBank contained over 220,000 entries of expressed sequence tags (ESTs) from plants, most publicly available plant ESTs are derived from vegetative tissues and relatively few ESTs are specifically derived from developing seeds. However, important morphogenetic processes are exclusively associated with seed and embryo development and the metabolism of seeds is tailored toward the accumulation of economically valuable storage compounds such as oil. Here we describe a new set of ESTs from Arabidopsis, which has been derived from 5- to 13-d-old immature seeds. Close to 28,000 cDNAs have been screened by DNA/DNA hybridization and approximately 10,500 new Arabidopsis ESTs have been generated and analyzed using different bioinformatics tools. Approximately 40% of the ESTs currently have no match in dbEST, suggesting many represent mRNAs derived from genes that are specifically expressed in seeds. Although these data can be mined with many different biological questions in mind, this study emphasizes the import of photosynthate into developing embryos, its conversion into seed oil, and the regulation of this pathway.
对来自不同植物的cDNA进行大规模单通道测序，为基因克隆、组织特异性基因表达评估、基于图谱克隆的标记以及基因组序列注释提供了丰富的资源。尽管截至2000年1月，GenBank中包含超过220,000条来自植物的表达序列标签（EST）条目，但大多数公开可用的植物EST来自营养组织，而专门来自发育种子的EST相对较少。然而，重要的形态发生过程仅与种子和胚胎发育相关，种子的代谢是为了积累经济上有价值的储存化合物，如油。在这里，我们描述了一组来自拟南芥的新EST，它们来自5至13日龄的未成熟种子。通过DNA/DNA杂交筛选了近28,000个cDNA，并使用不同的生物信息学工具生成并分析了约10,500个新的拟南芥EST。目前约40%的EST在dbEST中没有匹配项，这表明许多EST代表了在种子中特异性表达的基因衍生的mRNA。尽管这些数据可以从许多不同的生物学问题角度进行挖掘，但本研究强调了光合产物进入发育中的胚胎、其转化为种子油以及该途径的调控。
