Focks N, Benning C
Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, 14195 Berlin, Germany.
Plant Physiol. 1998 Sep;118(1):91-101. doi: 10.1104/pp.118.1.91.
During oil deposition in developing seeds of Arabidopsis, photosynthate is imported in the form of carbohydrates into the embryo and converted to triacylglycerols. To identify genes essential for this process and to investigate the molecular basis for the developmental regulation of oil accumulation, mutants producing wrinkled, incompletely filled seeds were isolated. A novel mutant locus, wrinkled1 (wri1), which maps to the bottom of chromosome 3 and causes an 80% reduction in seed oil content, was identified. Wild-type and homozygous wri1 mutant plantlets or mature plants were indistinguishable. However, developing homozygous wri1 seeds were impaired in the incorporation of sucrose and glucose into triacylglycerols, but incorporated pyruvate and acetate at an increased rate. Because the activities of several glycolytic enzymes, in particular hexokinase and pyrophosphate-dependent phosphofructokinase, are reduced in developing homozygous wri1 seeds, it is suggested that WRI1 is involved in the developmental regulation of carbohydrate metabolism during seed filling.
在拟南芥发育中的种子进行油脂沉积过程中，光合产物以碳水化合物的形式被转运到胚中，并转化为三酰甘油。为了鉴定该过程所必需的基因，并研究油脂积累发育调控的分子基础，分离出了产生皱缩、未完全充实种子的突变体。鉴定出一个新的突变位点——皱缩1（wri1），它位于第3号染色体的末端，导致种子油含量降低80%。野生型和纯合wri1突变体的幼苗或成熟植株没有明显差异。然而，发育中的纯合wri1种子在将蔗糖和葡萄糖掺入三酰甘油方面存在缺陷，但以更高的速率掺入丙酮酸和乙酸盐。由于在发育中的纯合wri1种子中几种糖酵解酶的活性，特别是己糖激酶和焦磷酸依赖性磷酸果糖激酶的活性降低，表明WRI1参与种子充实过程中碳水化合物代谢的发育调控。
