Ali M A, Jones J K
Department of Agricultural Botany, University of Reading, Reading, England, U.K.
Indian J Exp Biol. 2000 Jun;38(6):593-7.
Culture of isolated microspores and of anthers on media containing IAA directed free microspore development to an embryogenic pathway in C. olitorius. The first division of microspores on transfer to culture media was symmetrical in contrast to the asymmetrical division seen in normal development in vivo. Initially, 10-30% microspores divided symmetrically, but only 0.2-1% of the dividing microspores continued dividing and produced multicellular microcalli. About 30% of these microcalli produced callus but only on medium with 2.0 mg/L zeatin and 0.1 mg/L IAA. Incubation in the dark at temperatures of 35 degrees C for 1 day and then 25 degrees C was found effective for induction of first embryonic division in Corchorus. The frequency of microspore callus formation was higher on medium containing either 3% or 5% sucrose. Addition of colchicine and addition of activated charcoal to the above medium did not enhance microspore division in Corchorus olitorius. On transfer to different media most calli produced roots but regeneration of shoots and embryos was not induced.
在含有吲哚乙酸（IAA）的培养基上对分离出的小孢子和花药进行培养，可引导黄麻的游离小孢子发育进入胚胎发生途径。与体内正常发育中所见的不对称分裂相反，转移到培养基上的小孢子的第一次分裂是对称的。最初，10%至30%的小孢子进行对称分裂，但只有0.2%至1%的分裂小孢子继续分裂并产生多细胞小愈伤组织。这些小愈伤组织中约30%产生愈伤组织，但仅在含有2.0毫克/升玉米素和0.1毫克/升吲哚乙酸的培养基上。发现在35摄氏度黑暗中培养1天然后在25摄氏度下培养对诱导黄麻的第一次胚胎分裂有效。在含有3%或5%蔗糖的培养基上小孢子愈伤组织形成的频率更高。向上述培养基中添加秋水仙碱和活性炭并未增强黄麻小孢子的分裂。转移到不同培养基上后，大多数愈伤组织产生根，但未诱导出芽和胚的再生。
