彭氏变形杆菌12、13、37和44菌株粗糙型脂多糖碳水化合物主链的结构

The structure of the carbohydrate backbone of the rough type lipopolysaccharides from Proteus penneri strains 12, 13, 37 and 44.

作者信息

Vinogradov Evgeny, Sidorczyk Zygmunt

机构信息

Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, Ont., Canada K1A 0R6.

出版信息

Carbohydr Res. 2002 Apr 30;337(9):835-40. doi: 10.1016/s0008-6215(02)00037-x.

DOI:10.1016/s0008-6215(02)00037-x

PMID:11996837

Abstract

The following structure of the lipid A-core backbone of the rough type lipopolysaccharides (LPS) from Proteus penneri strains 12, 13, 37, and 44 was determined using NMR and mass spectroscopy and chemical analysis of the oligosaccharides obtained by mild-acid hydrolysis, alkaline O,N-deacylation, O-deacylation with hydrazine, and deamination of the LPSs:where K=H, R=PEtN, R(1)=alpha-Hep-(1-->2)-alpha-DDHep, and R(2)=alpha-GalN (strains 12 and 13) or beta-GlcNAc-(1-->4)-alpha-GlcN (strains 37 and 44). LPS from each strain contained several structural variants. LPS from strain 12 contained a variant with R(1)=alpha-DDHep, whereas LPS from strains 13, 37, and 44 contained structures with K=amide of beta-GalA with putrescine or spermidine. The phosphate group at O-1 of the alpha-GlcN residue in the lipid part was partially substituted with Ara4N.

摘要

利用核磁共振、质谱以及对通过温和酸水解、碱性O,N-脱酰基、用肼进行O-脱酰基和脂多糖脱氨所得到的寡糖进行化学分析，确定了彭氏变形杆菌菌株12、13、37和44的粗糙型脂多糖（LPS）的脂质A核心骨架的以下结构：其中K = H，R = PEtN，R(1) = α-Hep-(1→2)-α-DDHep，且R(2) = α-GalN（菌株12和13）或β-GlcNAc-(1→4)-α-GlcN（菌株37和44）。每个菌株的LPS都包含几种结构变体。菌株12的LPS包含一种R(1) = α-DDHep的变体，而菌株13、37和44的LPS包含K = β-GalA与腐胺或亚精胺的酰胺的结构。脂质部分α-GlcN残基O-1处的磷酸基团被Ara4N部分取代。