Drago L, Mombelli B, De Vecchi E, Bonaccorso C, Fassina M C, Gismondo M R
Laboratory of Clinical Microbiology, Department of Preclinical Science and L. Sacco Teaching Hospital, University of Milan, L.I.T.A. Vialba, Via G.B. Grassi 74, 20157 Milan, Italy.
Int J Antimicrob Agents. 2000 Dec;16(4):545-7. doi: 10.1016/s0924-8579(00)00296-x.
The preliminary results of a study to show the possibility that Candida albicans can internalize into epithelial cells are reported. The study was performed on buccal, vaginal and HeLa cells. Buccal and vaginal cells, at a concentration of 5 x 10(4) cells/ml and HeLa monolayers were incubated for 2, 3 and 4 h with 10(5) colony forming units of a Candida albicans isolate. After incubation, non-internalised yeasts were eliminated and samples were processed for examination by Scanning Electron Microscopy. Results suggest that receptor-mediated endocytosis could be involved in the pathogenesis of recurrent oral and vaginal infections. This phenomenon could represent an interesting experimental model to testing drug interference in the development of therapeutic strategies against C. albicans infections.
一项旨在证明白色念珠菌可内化进入上皮细胞可能性的研究的初步结果已被报道。该研究在颊细胞、阴道细胞和HeLa细胞上进行。将浓度为5×10⁴个细胞/毫升的颊细胞和阴道细胞以及HeLa单层细胞与一株白色念珠菌分离株的10⁵个菌落形成单位一起孵育2、3和4小时。孵育后，去除未内化的酵母，对样本进行处理以通过扫描电子显微镜检查。结果表明，受体介导的内吞作用可能参与复发性口腔和阴道感染的发病机制。这种现象可能代表一个有趣的实验模型，用于测试药物对针对白色念珠菌感染的治疗策略开发的干扰作用。
