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[成年蝾螈眼睛再生过程中乳酸脱氢酶同工酶的电泳及免疫荧光研究]

[Electrophoretic and immunofluorescent study of lactate dehydrogenase isoenzymes during eye regeneration in adult tritons].

作者信息

Mitashov V I, Korochkin L I, Serov O L, Poznakhirkina N A

出版信息

Ontogenez. 1979;10(3):293-7.

PMID:111201
Abstract

The spectrum of LDH isozymes was studied at the successive stages of retinal regeneration from the pigment epithelium and lens cells from the iris margin in the adults Pleurodeles waltlii. The combination of two methods, electrophoresis and immunofluorescence, has revealed the slow and rapid LDH isozymes with different intensity of histochemical staining in cells of the tissues under study (pigment epithelium, retina, iris and lens). During the regeneration the spectra of LDH isozymes peculiar to the pigment epithelium and iris and characterized by the predominance of slow forms were substituted by those peculiar to the retina and iris and characterized by the predominance of rapid forms. The rearrangement is realized in the proliferative phase during the transformation of one cell type into another.

摘要

在成年有肋蝾螈视网膜从色素上皮和虹膜边缘晶状体细胞再生的连续阶段,研究了乳酸脱氢酶(LDH)同工酶谱。电泳和免疫荧光两种方法相结合,揭示了所研究组织(色素上皮、视网膜、虹膜和晶状体)细胞中具有不同组织化学染色强度的慢速和快速LDH同工酶。在再生过程中,以慢速形式为主的色素上皮和虹膜特有的LDH同工酶谱被以快速形式为主的视网膜和虹膜特有的同工酶谱所取代。这种重排是在一种细胞类型向另一种细胞类型转变的增殖期实现的。

相似文献

1
[Electrophoretic and immunofluorescent study of lactate dehydrogenase isoenzymes during eye regeneration in adult tritons].[成年蝾螈眼睛再生过程中乳酸脱氢酶同工酶的电泳及免疫荧光研究]
Ontogenez. 1979;10(3):293-7.
2
[Lactate dehydrogenase isoenzymes during regeneration of the lens and retina in adult tritons].[成年蝾螈晶状体和视网膜再生过程中的乳酸脱氢酶同工酶]
Ontogenez. 1974;5(5):513-7.
3
[Proliferation of the cells of iris and pigment epithelium of the retina in the early periods after removal of the lens and iris in Spanish newts].[西班牙蝾螈晶状体和虹膜摘除术后早期虹膜及视网膜色素上皮细胞的增殖]
Ontogenez. 1976;7(2):141-7.
4
[Reutilization of labelled DNA precursors by the iris cells during the regeneration of the lens and retina in newts].
Ontogenez. 1976;7(1):64-9.
5
Proliferation of cells of pigmentary epithelium of retina in adult newts at late regeneration stages after retinectomy.视网膜切除术后成年蝾螈视网膜色素上皮细胞在再生后期的增殖。
Sov J Dev Biol. 1975 Jan;5(1):70-3.
6
Cell cycle parameters in dedifferentiating iris epithelial cells.去分化虹膜上皮细胞中的细胞周期参数。
J Embryol Exp Morphol. 1975 Oct;34(2):497-510.
7
[Melanin granule metabolism in the iris and retinal pigment epithelium in adult tritions after completion of eye regeneration].[成年蝾螈眼睛再生完成后虹膜和视网膜色素上皮中的黑色素颗粒代谢]
Ontogenez. 1978;9(2):183-8.
8
Regeneration of the retina in the adult newt, Triturus cristatus, following surgical division of the eye by a post-limbal incision.
J Exp Zool. 1977 Apr;200(1):41-54. doi: 10.1002/jez.1402000107.
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[Muscarinic-acetylcholine and beta-adrenaline receptors of the retinal and pigment epithelium cells during eye regeneration in tritons].
Izv Akad Nauk SSSR Biol. 1991 Jan-Feb(1):13-23.
10
[Radioautographic study of cell proliferation in the pigment epithelium of tritons after transplantation into the cavity of a lens-free eye].[将蝾螈色素上皮移植到无晶状体眼房后细胞增殖的放射自显影研究]
Ontogenez. 1984 Jan-Feb;15(1):49-54.

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