Wiedenmann J, Elke C, Spindler K D, Funke W
Abteilungen Okologie und Morphologie der Tiere (Bio III) and Allgemeine Zoologie und Endokrinologie (Bio I), Universität Ulm, 89069 Ulm, Germany.
Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14091-6. doi: 10.1073/pnas.97.26.14091.
We characterize two green fluorescent proteins (GFPs), an orange fluorescent protein, and a nonfluorescent red protein isolated from the sea anemone Anemonia sulcata. The orange fluorescent protein and the red protein seem to represent two different states of the same protein. Furthermore, we describe the cloning of a GFP and a nonfluorescent red protein. Both proteins are homologous to the GFP from Aequorea victoria. The red protein is significantly smaller than other GFP homologues, and the formation of a closed GFP-like beta-can is not possible. Nevertheless, the primary structure of the red protein carries all features necessary for orange fluorescence. We discuss a type of beta-can that could be formed in a multimerization process.
我们对从沟迎风海葵中分离出的两种绿色荧光蛋白(GFP)、一种橙色荧光蛋白和一种非荧光红色蛋白进行了表征。橙色荧光蛋白和红色蛋白似乎代表了同一种蛋白的两种不同状态。此外,我们描述了一种GFP和一种非荧光红色蛋白的克隆。这两种蛋白均与维多利亚多管水母的GFP同源。红色蛋白明显小于其他GFP同源物,无法形成封闭的GFP样β桶结构。然而,红色蛋白的一级结构具备产生橙色荧光所需的所有特征。我们讨论了一种可能在多聚化过程中形成的β桶结构类型。