乳链菌肽会消耗分枝杆菌中的三磷酸腺苷（ATP）和质子动力。
Nisin depletes ATP and proton motive force in mycobacteria.
作者信息
Chung H J, Montville T J, Chikindas M L
机构信息
Cook College, Department of Food Science, Rutgers--The State University of New Jersey, New Brunswick, NJ 08901-8520, USA.
出版信息
Lett Appl Microbiol. 2000 Dec;31(6):416-20. doi: 10.1046/j.1472-765x.2000.00840.x.
This study examined the inhibitory effect of nisin and its mode of action against Mycobacterium smegmatis, a non-pathogenic species of mycobacteria, and M. bovis-Bacill Carmette Guerin (BCG), a vaccine strain of pathogenic M. bovis. In agar diffusion assays, 2.5 mg ml(-1) nisin was required to inhibit M. bovis-BCG. Nisin caused a slow, gradual, time- and concentration-dependent decrease in internal ATP levels in M. bovis-BCG, but no ATP efflux was detected. In mycobacteria, nisin decreased both components of proton motive force (membrane potential, Delta Psi and Delta pH) in a time- and concentration-dependent manner. However, mycobacteria maintained their intracellular ATP levels during the initial time period of Delta Psi and Delta pH dissipation. These data suggest that the mechanism of nisin in mycobacteria is similar to that in food-borne pathogens.
本研究考察了乳酸链球菌素对耻垢分枝杆菌（一种非致病性分枝杆菌）以及牛分枝杆菌卡介苗（致病性牛分枝杆菌的一种疫苗株）的抑制作用及其作用模式。在琼脂扩散试验中，需要2.5 mg/ml的乳酸链球菌素来抑制牛分枝杆菌卡介苗。乳酸链球菌素使牛分枝杆菌卡介苗的内部ATP水平缓慢、逐渐地呈时间和浓度依赖性下降，但未检测到ATP外流。在分枝杆菌中，乳酸链球菌素使质子动力势的两个组成部分（膜电位，ΔΨ和ΔpH）呈时间和浓度依赖性下降。然而，在ΔΨ和ΔpH耗散的初始时间段内，分枝杆菌维持其细胞内ATP水平。这些数据表明，乳酸链球菌素在分枝杆菌中的作用机制与在食源性病原体中的作用机制相似。
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