Miller Stephen, Masuya Hayato, Zhang Jian, Walsh Emily, Zhang Ning
Department of Plant Biology & Pathology, 201 Foran Hall, 59 Dudley Road, Rutgers University, New Brunswick, New Jersey, 08901, United States of America.
Tohoku Research Center of Forestry & Forest Products Research Institute, 92-25 Nabeyashiki, Shimi-Kuriyagawa, Morioka, Iwate 020-0123, Japan.
PLoS One. 2016 Apr 20;11(4):e0154030. doi: 10.1371/journal.pone.0154030. eCollection 2016.
Cornus species (dogwoods) are popular ornamental trees and important understory plants in natural forests of northern hemisphere. Dogwood anthracnose, one of the major diseases affecting the native North American Cornus species, such as C. florida, is caused by the fungal pathogen Discula destructiva. The origin of this fungus is not known, but it is hypothesized that it was imported to North America with its host plants from Asia. In this study, a TaqMan real-time PCR assay was used to detect D. destructiva in dried herbarium and fresh Cornus samples. Several herbarium specimens from Japan and China were detected positive for D. destructiva, some of which were collected before the first report of the dogwood anthracnose in North America. Our findings further support that D. destructiva was introduced to North America from Asia where the fungus likely does not cause severe disease.
山茱萸属植物（山茱萸）是北半球天然森林中常见的观赏树木和重要的林下植物。山茱萸炭疽病是影响北美本土山茱萸属物种（如佛罗里达山茱萸）的主要病害之一，由真菌病原体毁灭盘多毛孢引起。这种真菌的起源尚不清楚，但据推测它是随寄主植物从亚洲引入北美的。在本研究中，采用TaqMan实时荧光定量PCR检测法检测干燥标本和新鲜山茱萸样本中的毁灭盘多毛孢。从日本和中国采集的几份标本被检测出毁灭盘多毛孢呈阳性，其中一些标本是在北美首次报道山茱萸炭疽病之前采集的。我们的研究结果进一步支持了毁灭盘多毛孢是从亚洲引入北美的这一观点，在亚洲这种真菌可能不会引发严重病害。
