Petelin M, Pavlica Z, Ivanusa T, Sentjurc M, Skaleric U
Department of Oral Medicine and Periodontology, Faculty of Medicine, University of Ljubljana, Slovenia.
J Clin Periodontol. 2000 Dec;27(12):918-25. doi: 10.1034/j.1600-051x.2000.027012918.x.
BACKGROUND, AIMS: The purpose of this study was to evaluate the influence of oxygen free radical scavengers on periodontal inflammation and healing process.
Experimental periodontitis was induced by elastic ligatures around premolars (P2, P3, P4) and 1st molars (M1) in the upper and lower jaws of 15 beagle dogs. 9 months after the beginning of the experiment, the ligatures were removed. After 3 weeks of stabilization period, all teeth were supragingivally scaled. The animals were divided into 3 groups of 5 dogs. The 1st group received a liposome-encapsulated superoxide dismutase (SOD), the 2nd group a liposome-encapsulated catalase (CAT) and the 3rd group received both enzymes encapsulated in liposomes. 4 treatment modalities were tested in each group; i.e., supragingival scaling only (1), supragingival scaling and enzymes (2), supra- and sub-gingival scaling and root planing (3) and supra- and sub-gingival scaling and root planing with subgingival application of enzymes (4). Enzymes were delivered subgingivally on a daily basis for a period of 6 weeks. Gingival index (GI), probing depth (PD), clinical attachment level (CAL), radiographic analysis and the histological evaluation were performed.
Around the teeth with scaling and root planing followed by the application of SOD, the greatest suppression of gingival inflammation (GI = 1.8 +/- 0.1 before versus GI = 1.2 +/- 0.2 after treatment) (p<0.003), the smallest size of connective tissue infiltrate (5.5 +/- 4.3%) (p<0.01), the greatest reduction of PD (PD= 3.2 +/- 1.0 mm before versus 2.00.7 mm after treatment) (p<0.001), and gain of CAL (CAL=3.0 +/- 1.7 mm before versus CAL=2.4 +/- 1.1 mm after treatment) (p<0.001) were observed. In addition, radiographic analysis showed the greatest alveolar bone apposition in the group of teeth treated with scaling and root planing followed by subgingival application of SOD or both enzymes (p<0.001).
In conclusion, we demonstrated that scaling and root planing with subgingival application of liposome-encapsulated SOD suppress peridontal inflammation on experimentally induced periodontitis in beagle dogs.
背景、目的：本研究旨在评估氧自由基清除剂对牙周炎症及愈合过程的影响。
在15只比格犬的上下颌前磨牙（P2、P3、P4）和第一磨牙（M1）周围用弹性结扎丝诱导实验性牙周炎。实验开始9个月后，去除结扎丝。经过3周的稳定期后，对所有牙齿进行龈上洁治。将动物分为3组，每组5只犬。第一组接受脂质体包裹的超氧化物歧化酶（SOD），第二组接受脂质体包裹的过氧化氢酶（CAT），第三组接受两种酶均包裹在脂质体中的制剂。每组测试4种治疗方式，即仅龈上洁治（1）、龈上洁治加酶（2）、龈上和龈下洁治及根面平整（3）以及龈上和龈下洁治及根面平整并在龈下应用酶（4）。酶每天龈下给药，持续6周。进行牙龈指数（GI）、探诊深度（PD）、临床附着水平（CAL）、影像学分析和组织学评估。
在进行洁治和根面平整并应用SOD的牙齿周围，观察到牙龈炎症的最大抑制（治疗前GI = 1.8±0.1，治疗后GI = 1.2±0.2）（p<0.003），结缔组织浸润的最小尺寸（5.5±4.3%）（p<0.01），PD的最大降低（治疗前PD = 3.2±1.0 mm，治疗后2.0±0.7 mm）（p<0.001），以及CAL的增加（治疗前CAL = 3.0±1.7 mm，治疗后CAL = 2.4±1.1 mm）（p<0.001）。此外，影像学分析显示，在进行洁治和根面平整并在龈下应用SOD或两种酶的牙齿组中，牙槽骨的附着最多（p<0.001）。
总之，我们证明在比格犬实验性诱导的牙周炎中，龈下应用脂质体包裹的SOD进行洁治和根面平整可抑制牙周炎症。
